FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777881
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGCCCCTCACACTTTTGGCCAGGGGACCAGCCTGCAGATCAAACGAACTGTGGCTGCACCAT116610.6558931672029793No Hit
CTATAATATTGCTGACAGTAATAAAGTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC113730.6396941077608681No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106170.5971715767253264No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT83050.4671291273150453No Hit
GTTTTATACACCTCCAATGATAGGAACTACTTAGCTTGGTACCAGCAGAA71120.40002677344546683No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59010.3319119783607564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54600.30710716859002374No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52940.29777021071714027No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT51460.2894456940593887No Hit
GCTTAGGAGGCTGTCCTACTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCATTGGAGGTGTATAAAACACT46520.26165980737743416No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45070.2535040309222046No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44310.2492292791249808No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43540.24489828059358304No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40610.2284179874806019No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39390.22155588591137426No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37900.2131751225194487No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37850.21289388884857874No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37700.2120501878359688No Hit
TTATTACTGTCAGCAATATTATAGTGCCCCTCACACTTTTGGCCAGGGGA35730.20096958120369135No Hit
CAGTAATAAAGTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA35440.19933842591264547No Hit
CTTTTGGCCAGGGGACCAGCCTGCAGATCAAACGAACTGTGGCTGCACCA34510.19410747963446373No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA34270.1927575580142878No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33860.1904514419131539No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33100.18617669011593013No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32680.18381432728062227No Hit
GTAATAAAGTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA32020.18010204282513848No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29330.1649716713323333No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACC29200.1642404637880713No Hit
AAGTAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA29080.16356550297798336No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28890.1624968150286774No Hit
CTTTATTACTGTCAGCAATATTATAGTGCCCCTCACACTTTTGGCCAGGGGACCAGCCTGCAGATCAAACGAA28790.16193434768693743No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28010.15754710242136566No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACCCCG27390.15405980490257784No Hit
GGCCAAAAGTGTGAGGGGCACTATAATATTGCTGACAGTAATAAAGTGCC26730.15034752044709404No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26130.1469727163966542No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA26120.1469164696624802No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA25830.1452853143714343No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCACTTTATTACTGTCAGCAATATTATAGTGCC25760.14489158723221635No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25490.1433729254095184No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA25390.14281045806777845No Hit
GTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACCCCGGATTCCCGGGT25150.1414605364476025No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25020.14072932890334056No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24930.14022310829577458No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24310.13673581077698677No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA23920.13454218814420088No Hit
GTGCAGCCACAGTTCGTTTGATCTGCAGGCTGGTCCCCTGGCCAAAAGTG23510.132236072043067No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22880.1286925277901052No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22640.12734260616992926No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTT22450.1262739182206233No Hit
GTCCTACTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATCATTGGAGGTG22350.12571145087888336No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21970.12357407498027147No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21870.12301160763853149No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21720.12216790662592154No Hit
GGCTGAGGATGTGGCACTTTATTACTGTCAGCAATATTATAGTGCCCCTCACACTTTTGGCCAGGGGACCAGC21410.12042425786652762No Hit
GGATCTCTGGTGCGTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG21160.1190180895121777No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21060.11845562217043773No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20600.11586827239843386No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG20310.11423711710738796No Hit
CCTCCAATGATAGGAACTACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGCTGCTCATTTACTGGG20140.11328092262643001No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20080.11294344222138601No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19900.11193100100625407No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTACTTTCTGCTGGTACCAAGCTA19900.11193100100625407No Hit
GTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG19490.10962488490512019No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19430.10928740450007622No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19110.1074875090065083No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19000.10686879493059434No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18970.10670005472807235No Hit
GTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18870.10613758738633237No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCAGGCTGGTCCCCTGGCCAAAAGTGTGAGGGGCA18860.10608134065215838No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18820.1058563537154624No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18730.10535013310789643No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTACTTTCTGCTGGTA18490.10400021148772048No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18360.10326900394345853No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18220.10248154966502258No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17950.10096288784232466No Hit
GAACTACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGCTGC17880.10056916070310666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA200.0059933954.5079662
GGTATCA22900.042.9365431
GTGGTAT6350.041.3619081
TGGTATC6550.038.558852
GTCGCGC350.001508509738.53637719
GTATCAA59500.038.483241
GACAGGT4550.034.0428661
TATCAAC68450.033.256562
CGCAATG550.003190912533.0790869
ATCAACG70600.032.1951873
TCAACGC70950.032.035474
CAACGCA72000.031.6132725
AACGCAG72550.031.5128446
ACGCAGA75450.030.2569857
CGCAGAG76550.029.777388
AGAGTAC77800.029.2099311
CAGAGTA78650.028.93868410
GCAGAGT78800.028.9271379
CCGAGTT957.5443386E-626.53091859
TTAACGG1457.9671736E-1025.89614735
Produced by FastQC (version 0.11.5)