FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982123
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCTCCTTCGGGCGCTTATGGCCAGGGGACCAAGCTGG71740.7304584049044773No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC60550.6165215558540019No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50570.5149049558965628No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44630.45442373307620326No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTAATCTATAAGGCGT38410.39109154352357084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33090.3369231756103869No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31400.31971555497631154No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28860.2938532139049793No Hit
CTTATGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA25120.2557724439810492No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23880.24314673416669808No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22890.2330665303633048No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22190.22593911353262272No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22040.22441180992604795No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21830.22227358487684332No Hit
GGCCATAAGCGCCCGAAGGAGAATAACTATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGG21810.22206994439596667No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21320.21708075261448923No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTA20290.2065932678493427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20030.2039459415979465No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19700.20058587366348204No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18670.19009838889833555No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17980.18307279230809176No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17930.18256369110590018No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17820.1814436684610787No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA17770.18093456725888712No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17330.17645447667960124No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG17260.17574173499653303No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA17140.17451989211127325No Hit
CAGTATAATAGTTATTCTCCTTCGGGCGCTTATGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA17080.17390897066864333No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17030.17339986946645175No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16680.16983616105111068No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTAATCTATAAGGCGTCTA16560.16861431816585093No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15980.16270874422042858No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA15830.16118144061385387No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15540.1582286536411427No Hit
TCTATAAGGCGTCTACTTTAAAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15410.1569049905154446No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15130.15405402378317176No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14650.1491666522421326No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14230.14489020214372333No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14010.1426501568540804No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13690.13939190916005428No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGC13450.13694822338953472No Hit
CCTTATAGATTAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA13240.13480999834033008No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12950.1318572113676189No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12820.13053354824192082No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCATAAGCGCCCGAAGGAGAATAACTATTATA12760.12992262679929092No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12660.12890442439490776No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12650.12880260415446945No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12350.12574799694132No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCTCCTTCGGGCGCTTATGGCCAGGGGACCAAGCTGGAGATCAAA12330.12554435646044335No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC12300.1252388957391284No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12130.12350795165167704No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11900.12116608612159577No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11780.11994424323633597No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11610.1182132991488846No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11590.11800965866800797No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA11550.11760237770625472No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG11300.11505687169529681No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11190.11393684905047535No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11180.11383502881003704No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10960.1115949835203941No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10940.11139134303951746No Hit
GTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG10910.1110858823182025No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10860.11057678111601092No Hit
TAGTTATTCTCCTTCGGGCGCTTATGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10710.1090494775094362No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10600.10792945486461471No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10570.10762399414329976No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10560.10752217390286145No Hit
ACCTTGATGGGACCCCACTTTTTAAAGTAGACGCCTTATAGATTAGGAGGTTAGGGGCTTTCCCTGGTTTCTG10130.10314390356401387No Hit
GATTAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC9960.10141295947656251No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG9940.10120931899568587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG251.6408831E-460.4305667
TCGCACC252.4011513E-455.9511547
CCTAACG200.00808609750.5306472
AAGTCGT356.663161E-445.49503368
CGGAAAT350.001467722238.7501930
GTATCAA23750.037.451591
TGCACCA28100.035.28711370
ATAAGTC450.003222827633.0107966
TATCAAC27950.031.577892
TCAACGC29200.030.3430084
ATCAACG29200.030.2276343
CAACGCA29700.029.944095
GCAACGA450.00519405529.9440886
TTTCGGT803.160927E-629.66811630
CAGTTCG17900.029.5439270
AACGCAG30500.029.269126
CTTATAT606.2312715E-428.074013
ACGCAGA32300.027.6380237
CGCAGAG32600.027.5903558
AGAGTAC33200.027.39194911
Produced by FastQC (version 0.11.5)