FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799599
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31470.3935722781043999No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30110.3765637525809812No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTGCAGTGAAGCTGTCTGTACATGAGCTGCAGTAATAAT27620.3454231433506045No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24470.30602839673386284No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24270.30352714298041894No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23570.29477275484336524No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG21680.2711359068733203No Hit
GATTATTACTGCAGCTCATGTACAGACAGCTTCACTGCAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGT20850.260755703796528No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18300.2288647184401181No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18200.22761409156339618No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17620.2203604556784088No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16790.20998025260161654No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16720.2091048137879112No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16700.20885468841256677No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTGCAGTGAAG16150.20197624059059605No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16090.2012258644645629No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15330.19172110020147598No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATGT14540.18184114787537253No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTCATAATTATGTCTCATGGTATCAACAACACCCAGGCAAAGC14090.17621332693012373No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT13710.1714609447985803No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13620.17033538060953052No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13540.16933487910815295No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTCATAATTATGTCTCATGGTATCAACAACA13420.16783412685608662No Hit
GTCTGTACATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCC13170.16470755966428172No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13080.16358199547523197No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG12750.1594549267820495No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12470.15595317152722804No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG11970.14970003714361824No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11950.14944991176827382No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11710.14644840726414116No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11700.14632334457646895No Hit
GGATGAGGCTGATTATTACTGCAGCTCATGTACAGACAGCTTCACTGCAGTCTTCGGAACTGGGACCAAGGTCAC11570.1446975296367304No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCCTCAGGGAGAATTGGTTCTTGGACGTGTCTACTGA11540.14432234157371382No Hit
GTCTAGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATG11460.14332184007233628No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11130.1391947713791538No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTGCAGTGAAGCTGTCTGTACATGAGCTG11010.13769401912708745No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCCTCAGGGAGAATTGGTTCTTGGACGTGTCTA10770.13469251462295476No Hit
TGTACAGACAGCTTCACTGCAGTCTTCGGAACTGGGACCAAGGTCACCGT10340.12931481905305034No Hit
TAATTATGTCTCATGGTATCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAGCGGCC10320.12906469367770596No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10260.12831431755167277No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT10110.12643837723658985No Hit
GTTCCGAAGACTGCAGTGAAGCTGTCTGTACATGAGCTGCAGTAATAATC9930.12418724885849032No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCTAGACTTGGAGCCAGAGAAG9810.12268649660642397No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9660.12081055629134105No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9610.12018524285298006No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9480.11855942791324152No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC9450.11818423985022491No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9440.11805917716255274No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT9250.11568298609678102No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG9250.11568298609678102No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAGCGGCCCTCGGGGGTCCCTGATCG9190.11493260997074783No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8860.11080554127756538No Hit
TTGCTAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCCGAGGGCCGCTT8860.11080554127756538No Hit
GCCCTCGGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTAGCAACACGG8850.11068047858989319No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8810.1101802278392044No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT8770.10967997708851561No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8630.10792909946110488No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8620.10780403677343268No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8340.10430228151861119No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8320.10405215614326681No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8290.10367696808025022No Hit
GTACATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGA8240.10305165464188926No Hit
GTATCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA8080.10105065163913411No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC8010.10017521282542875No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATGTACAGACAGCTTCACTG8000.10005015013775656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)