FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799599
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31770.3973241587345657No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT29020.3629319196247119No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTGCAGTGAAGCTGTCTGTACATGAGCTGCAGTAATAAT27310.3415462000327664No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26550.33204143576967954No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25290.3162835371229829No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23890.2987747608488755No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22680.28364217564053984No Hit
GATTATTACTGCAGCTCATGTACAGACAGCTTCACTGCAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGT21670.271010844185648No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG21310.26650858742944905No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17730.22173614524280297No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17490.21873464073867027No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16700.20885468841256677No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC16060.20085067640154627No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15570.1947226047056087No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15400.19259653901518137No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTGCAGTGAAG15050.18821934494665452No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14940.18684365538226036No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14650.1832168374397667No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTCATAATTATGTCTCATGGTATCAACAACACCCAGGCAAAGC14270.17846445530822325No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATGT14190.17746395380684568No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14090.17621332693012373No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13930.17421232392736857No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTCATAATTATGTCTCATGGTATCAACAACA13510.1689596910451364No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12660.15832936259299973No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12270.1534519177737841No Hit
GGATGAGGCTGATTATTACTGCAGCTCATGTACAGACAGCTTCACTGCAGTCTTCGGAACTGGGACCAAGGTCAC12230.15295166702309532No Hit
GTCTGTACATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCC12140.15182610283404555No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11750.14694865801482992No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT11290.14119577438190895No Hit
TAATTATGTCTCATGGTATCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAGCGGCC11210.14019527288053138No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCCTCAGGGAGAATTGGTTCTTGGACGTGTCTA10840.13556795343666014No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10800.13506770268597135No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC10800.13506770268597135No Hit
GTCTAGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATG10640.13306669968321622No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTGCAGTGAAGCTGTCTGTACATGAGCTG10560.13206619818183865No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10460.13081557130511667No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCCTCAGGGAGAATTGGTTCTTGGACGTGTCTACTGA10340.12931481905305034No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG10290.12868950561468936No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10150.12693862798727862No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9890.12368699810780152No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9890.12368699810780152No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9750.12193612048039079No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT9310.11643336222281418No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAGCGGCCCTCGGGGGTCCCTGATCG9230.11543286072143663No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9130.11418223384471467No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT9080.11355692040635368No Hit
GTTCCGAAGACTGCAGTGAAGCTGTCTGTACATGAGCTGCAGTAATAATC8980.11230629352963174No Hit
TTGCTAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCCGAGGGCCGCTT8900.11130579202825415No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT8890.11118072934058196No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8710.10892960096248244No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8650.10817922483644926No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC8590.1074288487104161No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8520.10655340989671072No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT8430.10542784570766098No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC8400.10505265764464437No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8400.10505265764464437No Hit
GCCCTCGGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTAGCAACACGG8210.10267646657887265No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8180.10230127851585608No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8170.10217621582818388No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8120.10155090238982291No Hit
GTATCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA8120.10155090238982291No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8060.10080052626378973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.00791942150.796743
ATGGCAA1302.5852205E-740.84916770
CCGCTAT800.001628721637.93136670
ATCAACG25200.033.730113
TCAACGC25300.033.596794
GTGGTAT2450.033.1650771
AACGCAG25800.032.8164836
CAACGCA25850.032.753015
GACAGGT1500.031.598951
ACGCAGA26800.031.3392527
CCCATAG652.6395703E-531.2517091
CGCAGAG26950.031.0391628
AGAGTAC27650.030.00087711
CCGACTG1251.5279511E-1029.82502218
CAGAGTA27950.029.805610
GCAGAGT28500.029.3510639
ATACGTA1052.7956048E-829.0212611
ACCGTGC755.1856507E-528.30700555
AATGGCA1304.251542E-728.12363469
GAGTTTA2450.027.6375641
Produced by FastQC (version 0.11.5)