FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004689507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695129
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58610.3457553967869112No Hit
GTAATAGACGGCCGTGTCCGCGGCGCTCACATACGTCACCTCCAAGGAGAATTCATTCTTGGACTTGTCTAGTGA55740.32882453193827726No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53500.31561019839788007No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40740.24033569126597443No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39840.23502636082563627No Hit
GTCTATTACTGTGCGAGACGCGGCGGCGACAGCAACTCTCACCTCTATTTCTTTGACTACTGGGGCCGGGGAACC35050.2067689243709476No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34080.201046646007472No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33360.19679918165520144No Hit
GTAGGGGACAGGGTCACTATCACTTGCCGGGCAAGTCAGTACATCAGTAACTATTTAAATTGGTATCAGCAGAAA33280.19632724117161585No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33040.194911419720859No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32440.1913718660939669No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31050.18317190019166682No Hit
GACCTACATGTACCCCCGGGCGTTCGGCCAAGGGACCAAGGTGACAATCCGGCGAACTGTGGCTGCACCAT31040.18311290763121862No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28530.16830577495871996No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28330.1671259237497559No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG28170.1661820427825847No Hit
ATGTAGGTCTGTTGACAGTAGTAAATCGCAAGATCTTCAGGTTGCAGATTGTTGATGGTGAGAGTGAAGTTTGTC27970.16500219157362064No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27830.16417629572734582No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26820.15821804712207743No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26260.15491446373697812No Hit
GTCTCCACCCTCCCTGTCTGCATCTGTAGGGGACAGGGTCACTATCACTTGCCGGGCAAGTCAGTACATCAGTAA25820.15231879107725724No Hit
GTCTCTGGTGACTCCGTCAGCGGTGACACTTGGTGGACCTGGGTCCGCCA25360.14960513329663996No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25360.14960513329663996No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC24440.1441778177354054No Hit
GTGCAGCCACAGTTCGCCGGATTGTCACCTTGGTCCCTTGGCCGAACGCC24240.14299796652644134No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23980.14146415995478812No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23760.14016632362492767No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23340.13768863608610318No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23190.13680374767938017No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT23040.13591885927265712No Hit
GTACATCAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGGAAGCCCCTAGGATCCTGATCTATGGTTCATT22480.1326152758875578No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22340.131789380041283No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22080.13025557346962974No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22030.12996061066738873No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCCGGATTGTCACCTTGGTCC21940.12942967762335492No Hit
GATCTATGGTTCATTCACTTTGCAAACTGGGGTCCCATCAAGATTCACTGGCAGTGGATCTGGGACAAACTTCA20760.12246855549046709No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20660.12187862988598508No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20580.12140668940239946No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20330.11993187539119442No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20120.11869303162178217No Hit
AGTCAGTACATCAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGGA20010.11804411345685195No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19960.11774915065461095No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19860.11715922505012893No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19840.11704123992923253No Hit
GGGTCACTATCACTTGCCGGGCAAGTCAGTACATCAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGGAAG19810.11686426224788792No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19500.11503549287399367No Hit
GTAGTAAATCGCAAGATCTTCAGGTTGCAGATTGTTGATGGTGAGAGTGA19440.11468153751130444No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19440.11468153751130444No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGGTGGGTC19360.11420959702771884No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19080.1125578053351692No Hit
CACCTGCACTGTCTCTGGTGACTCCGTCAGCGGTGACACTTGGTGGACCT18950.11179090204934256No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18570.10954918475231089No Hit
ATTTACTACTGTCAACAGACCTACATGTACCCCCGGGCGTTCGGCCAAGGGACCAAGGTGACAATCCGGCGAA18350.10825134842245045No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18180.10724847489483101No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17760.10477078735600653No Hit
GTCTGCATCTGTAGGGGACAGGGTCACTATCACTTGCCGGGCAAGTCAGT17750.10471179479555834No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17720.10453481711421374No Hit
ACATGTACCCCCGGGCGTTCGGCCAAGGGACCAAGGTGACAATCCGGCGA17720.10453481711421374No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17660.10418086175152451No Hit
CAGTAATAGACGGCCGTGTCCGCGGCGCTCACATACGTCACCTCCAAGGA17550.1035319435865943No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17290.10199813701494104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)