FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695129
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59090.34858703968842486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58260.34369065717122416No Hit
GTAATAGACGGCCGTGTCCGCGGCGCTCACATACGTCACCTCCAAGGAGAATTCATTCTTGGACTTGTCTAGTGA55540.32764468072931324No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44310.2613960353459825No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41730.24617595475034645No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41390.24417020769510758No Hit
GTCTATTACTGTGCGAGACGCGGCGGCGACAGCAACTCTCACCTCTATTTCTTTGACTACTGGGGCCGGGGAACC34600.20411425915077852No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33710.1988639212708885No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32600.19231574706113813No Hit
GACCTACATGTACCCCCGGGCGTTCGGCCAAGGGACCAAGGTGACAATCCGGCGAACTGTGGCTGCACCAT30630.18069421265284236No Hit
GTAGGGGACAGGGTCACTATCACTTGCCGGGCAAGTCAGTACATCAGTAACTATTTAAATTGGTATCAGCAGAAA30170.17798055487222506No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29790.17573883757519337No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29170.17208129882740486No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27690.163350399881071No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27660.16317342219972641No Hit
GTCTCTGGTGACTCCGTCAGCGGTGACACTTGGTGGACCTGGGTCCGCCA27280.16093170490269473No Hit
ATGTAGGTCTGTTGACAGTAGTAAATCGCAAGATCTTCAGGTTGCAGATTGTTGATGGTGAGAGTGAAGTTTGTC27190.1604007718586609No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26980.15916192808924867No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25600.1510209547473968No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25410.14990009609888097No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25140.14830729696677952No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24890.14683248295557447No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24600.14512169870257663No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24330.14352889957047518No Hit
GTCTCCACCCTCCCTGTCTGCATCTGTAGGGGACAGGGTCACTATCACTTGCCGGGCAAGTCAGTACATCAGTAA24120.1422900558010629No Hit
GTGCAGCCACAGTTCGCCGGATTGTCACCTTGGTCCCTTGGCCGAACGCC23640.13945841289954924No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22860.1348569931845895No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22810.13456203038234849No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22690.13385411965697006No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22620.13344117173383266No Hit
GTACATCAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGGAAGCCCCTAGGATCCTGATCTATGGTTCATT21990.12972464042559595No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21900.1291937073815621No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21740.12824982641439087No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21700.12801385617259808No Hit
GGGTCACTATCACTTGCCGGGCAAGTCAGTACATCAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGGAAG21350.125949116556911No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21120.12459228766660237No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCCGGATTGTCACCTTGGTCC21010.12394336950167216No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20820.12282251085315632No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20810.1227635182927081No Hit
GATCTATGGTTCATTCACTTTGCAAACTGGGGTCCCATCAAGATTCACTGGCAGTGGATCTGGGACAAACTTCA20420.12046280843522822No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19530.11521247055533827No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19140.1129117606978584No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19080.1125578053351692No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGGTGGGTC18870.11131896156575695No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18820.11102399876351593No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18710.11037508059858572No Hit
GTAGTAAATCGCAAGATCTTCAGGTTGCAGATTGTTGATGGTGAGAGTGA18630.10990314011500009No Hit
GTCTGCATCTGTAGGGGACAGGGTCACTATCACTTGCCGGGCAAGTCAGT18590.10966716987320729No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18540.10937220707096629No Hit
ATTTACTACTGTCAACAGACCTACATGTACCCCCGGGCGTTCGGCCAAGGGACCAAGGTGACAATCCGGCGAA18190.1073074674552792No Hit
AGTCAGTACATCAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGGA18140.10701250465303822No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18140.10701250465303822No Hit
GGGGTACATGTAGGTCTGTTGACAGTAGTAAATCGCAAGATCTTCAGGTT18060.10654056416945258No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17650.1041218691910763No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17500.1032369807843533No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17250.10176216677314823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)