FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778632
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGATACA73010.4104840124320264No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT55100.30978864655533017No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC47300.2659347183678243No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTAGTCCCA46600.2619991094279199No Hit
GTCTATTACTGTGCGAGAGATTTTCTAATGACTTTTAACTGCTGGGGCCA42420.23849790175820518No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG42310.23787944892479165No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41930.23574297550027212No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC39920.22444215554426097No Hit
GATTATTACTGCAGCTCATTTGCAGGCACCAACAATTATGTCTTCGGAAGTGGGACTAAGGTCACCGTCCTAGGT39060.2196069788466642No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG39000.2192696409375295No Hit
GACCTAGGACGGTGACCTTAGTCCCACTTCCGAAGACATAATTGTTGGTGCCTGCAAATGAGCTGCAGTAATAAT35020.19689289296493037No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT32280.18148779511444751No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32150.1807568963113224No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31630.177833301098822No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC31220.17552815871973518No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31100.17485348290146585No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC30520.1715925497798308No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTAGTC30310.1704118670978595No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30090.1691749614310324No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTAGTCCCACTTCCGAAGACATAATTGTTGGTGCCTGCAAATGAGCTG29070.16344021697574315No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT28250.15882993221756947No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT27060.15213939701973203No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25900.1456175307764619No Hit
ACCTAGGACGGTGACCTTAGTCCCACTTCCGAAGACATAATTGTTGGTGC25710.14454929406420217No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATTT25640.14415573317021171No Hit
GTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGA25410.14286260451852886No Hit
CCTTAGTCCCACTTCCGAAGACATAATTGTTGGTGCCTGCAAATGAGCTGCAGTAATAATCAGCCTCATCCTCAG25410.14286260451852886No Hit
ACCACATACTACGCAGACTCTGTGAGGGGCCGATTCACCGTCTCCAGAGACAATGCCAAGAACTCACTGTATCTG25050.14083857706372088No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC24910.14005145527574No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAACA24850.13971411736660536No Hit
GGATGAGGCTGATTATTACTGCAGCTCATTTGCAGGCACCAACAATTATGTCTTCGGAAGTGGGACTAAGGTCAC23700.13324847410819102No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG23610.13274246724448902No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23370.1313931156079504No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23260.13077466277453684No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23160.13021243292597906No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC22760.127963513531748No Hit
CAATTATGTCTTCGGAAGTGGGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT22680.12751372965290178No Hit
GTACCTATACTATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGGCTGGAG22630.1272326147286229No Hit
GTATGTGGTAGTACTAGAACCCGTAATGGATGAAACCCACTCCAGCCCCTTCCCCGGAGCCTGGCGGACCCAGTT22390.12588326309208425No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21820.12267855295530498No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21760.1223412150461703No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21540.12110430937934322No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT21130.11879916700025639No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21130.11879916700025639No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20860.11728114640915041No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20430.11486355806035199No Hit
GGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAG20250.11385154433294802No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20060.11278330762068825No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19990.11238974672669781No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19740.1109841721053034No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC19650.1104781652416014No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT19480.10952237449905322No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATACTATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGG19060.10716100913511059No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA19030.10699234018054324No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18840.1059241034682835No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18700.10513698168030261No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT18350.10316917721035043No Hit
CAGTAATAGACAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGATA18080.10165115661924445No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17910.10069536587669625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)