FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778632
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63830.3588713123344233No Hit
GTAATAGACAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGATACA62620.3520683311668743No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC51220.2879741284312888No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49650.2791471198089318No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTAGTCCCA44210.24856181604738922No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43590.2450759909863311No Hit
GTCTATTACTGTGCGAGAGATTTTCTAATGACTTTTAACTGCTGGGGCCA42200.23726099609137807No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG39490.22202456719546257No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39430.22168722928632792No Hit
GATTATTACTGCAGCTCATTTGCAGGCACCAACAATTATGTCTTCGGAAGTGGGACTAAGGTCACCGTCCTAGGT38780.21803273527070244No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34790.1955997643132475No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC34220.1923950541764682No Hit
GACCTAGGACGGTGACCTTAGTCCCACTTCCGAAGACATAATTGTTGGTGCCTGCAAATGAGCTGCAGTAATAAT32220.18115045720531284No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31110.17490970588632163No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30910.1737852461892061No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28850.16220331130891605No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28760.16169730444521407No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTAGTCCCACTTCCGAAGACATAATTGTTGGTGCCTGCAAATGAGCTG28550.16051662176324277No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTAGTC28370.1595046080358388No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT28340.15933593908127147No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28330.15927971609641567No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27700.15573766805050174No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26900.15123982926203958No Hit
GTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGA25660.14426817913992326No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAACA24920.14010767826059578No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC24690.1388145496089129No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATTT24370.13701541409352805No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24200.13605962335097985No Hit
CCTTAGTCCCACTTCCGAAGACATAATTGTTGGTGCCTGCAAATGAGCTGCAGTAATAATCAGCCTCATCCTCAG23200.1304373248654022No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC22700.12762617562261333No Hit
GGATGAGGCTGATTATTACTGCAGCTCATTTGCAGGCACCAACAATTATGTCTTCGGAAGTGGGACTAAGGTCAC22490.12644549294064203No Hit
ACCACATACTACGCAGACTCTGTGAGGGGCCGATTCACCGTCTCCAGAGACAATGCCAAGAACTCACTGTATCTG22350.12565837115266115No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22320.12548970219809383No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22220.12492747234953605No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21630.12161031624304522No Hit
GTACCTATACTATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGGCTGGAG21580.12132920131876633No Hit
GTATGTGGTAGTACTAGAACCCGTAATGGATGAAACCCACTCCAGCCCCTTCCCCGGAGCCTGGCGGACCCAGTT21210.11924895087910259No Hit
ACCTAGGACGGTGACCTTAGTCCCACTTCCGAAGACATAATTGTTGGTGC20640.11604424074232332No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATACTATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGG20400.11469488910578467No Hit
CAATTATGTCTTCGGAAGTGGGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT20390.1146386661209289No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT20320.11424510522693847No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20200.11357042940866914No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19840.11154640195386116No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19610.11025327330217831No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19560.10997215837789942No Hit
GGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19430.10924125957477433No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG19170.10777946196852413No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA19000.10682367122597591No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18720.10524942765001417No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18330.10305673124063888No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18260.10266317034664843No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18250.10260694736179267No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT18070.10159493363438866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG20300.055.97875270
GGTATCA15250.043.4724271
GTGGTAT4050.041.1329771
TCGTCTC16850.037.96735820
GTATCAA40250.037.8408781
TGGTATC4450.036.671642
CGTCGTC17950.036.01475518
GTTCATT17550.035.7107334
GCAGATA18350.035.4324242
TCGTCGT18250.035.4197417
AGATACA18450.035.3273144
CAGATAC18400.035.0012843
TTGCAGA18750.034.9404440
GTCGTCT18650.034.482519
GTGAGTT19250.034.3725451
TGCAGAT19000.034.1694141
GTCTATA400.002790333733.9976651
AGTGAGT19600.033.67971850
CGTGTCG18950.033.54631813
GTCGTCG19350.033.4062116
Produced by FastQC (version 0.11.5)