FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252065
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGAGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGA61780.49342486212776493No Hit
GTGTATTACTGTGCGAGAACATATTACTATGATAGTAGTGGTTATGGGCC40220.3212293291482471No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37690.30102271048228324No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32350.258373167527245No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC29770.23776720857144No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24950.19927080463075No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24400.19487806144249697No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT23960.19136386689189458No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23920.19104439466002163No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23020.18385626944288036No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22280.17794603315323088No Hit
CAGTAATACACAGCCGAGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGA21900.17491104695043788No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21030.16796252590720132No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT20930.16716384532751893No Hit
CTCCTGCAGGGCCAGTCAGACTGTCAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT20240.16165294932771063No Hit
GTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCAGTGGCCCTGTT19590.15646152555977524No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19540.15606218526993407No Hit
GTATTACTGTGCGAGAACATATTACTATGATAGTAGTGGTTATGGGCCTT19520.1559024491539976No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19520.1559024491539976No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTCAGCAG19490.1556628449800929No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19480.15558297692212464No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19290.15406548382072816No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGAAGCAAC19070.15230838654542697No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18830.15039155315418928No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18820.15031168509622106No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18740.14967274063247515No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTTCTGGGGCTGGATCCG18660.14903379616872925No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18560.1482351155890469No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18390.14687735860358686No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18000.14376250434282564No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17870.1427242195892386No Hit
GTTTATTACTGTCAGCAGCGAAGCAACTTCGGCCCTGGGACCAAAGTGGA17640.14088725425596915No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17610.14064765008206442No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17400.13897042086473146No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17000.13577569854600202No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16950.13537635825616082No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16920.13513675408225612No Hit
CTATTATAGTGGGACCACTTACTACAACCCGTCCCTCAAGAGTCGAGTCA16720.13353939292289138No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCA16430.13122321924181252No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTCAGCAGCTACTTAGCCTGGTA16390.13090374700993956No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16240.12970572614041603No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA16220.12954599002447956No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15840.12651100382168656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15650.12499351072029008No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15590.12451430237248066No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15070.12036116335813238No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC14880.11884367025673587No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14800.11820472579298998No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14410.11508987153222876No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14110.11269382979318167No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14080.11245422561927695No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13870.11077699640194398No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13830.11045752417007104No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13610.10870042689476984No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA13510.10790174631508746No Hit
ACTTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCTCTAGACACGTCCAAGAATCAGTTCTCCCTGAAG13510.10790174631508746No Hit
GGACTGGAGTGGATTGGGAATATCTATTATAGTGGGACCACTTACTACAA13480.10766214214118276No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGAAATCAAAAGGCCCATAAC13360.10670372544556393No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAGTTGCTTCGCTGCTGACAGTAATAAACTG13210.10550570457604037No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13100.10462715593838978No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13050.1042278156485486No Hit
CTATAATAGATATTCCCAATCCACTCCAGTCCCTTCCCCGGGGGCTGGCGGATCCAGCCCCAGAAGTAATTACTA12700.10143243361966031No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC12630.10087335721388267No Hit
CCATTGTCCCTTGGCCCCAGAAATCAAAAGGCCCATAACCACTACTATCA12570.10039414886607324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT208.632124E-568.8089439
TCGTATT252.571902E-455.17978340
AATCGCG252.6859305E-454.6973136
CGCGTAT408.4255953E-751.606739
TAATATC408.908246E-751.19169635
TAGAGAT16950.050.74725370
CGTATGC4950.048.8657941
ATGCCGT5100.048.4071744
TCGTATG5000.046.90281340
GCCGTCT4950.045.8500146
ATATCGT306.546225E-445.66057237
ATCGCGT306.546225E-445.66057237
AATATCG451.9825493E-645.5810936
CTTATAC5850.045.560551
AATCTCG4750.045.34119436
TAATCTC4900.044.57508535
TATACAC6250.044.248183
ACGAGAC6150.043.8936822
GCTAATC5450.043.7084633
GTATGCC5550.043.6777242
Produced by FastQC (version 0.11.5)