FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252065
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59990.47912847975145056No Hit
GTAATACACAGCCGAGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGA58050.4636340765056127No Hit
GTGTATTACTGTGCGAGAACATATTACTATGATAGTAGTGGTTATGGGCC39610.3163573776121847No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38060.30397782862710804No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC27560.22011636776045973No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26280.20989325634052547No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24610.19655529065982996No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22660.18098101935602384No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21080.1683618661970425No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21050.16812226202313776No Hit
CTCCTGCAGGGCCAGTCAGACTGTCAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT20510.16380938689285302No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20290.1620522896175518No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT20110.16061466457412354No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20040.1600555881683459No Hit
GTATTACTGTGCGAGAACATATTACTATGATAGTAGTGGTTATGGGCCTT19690.15726020613945763No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19460.1554232408061882No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19120.15270772683526815No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18700.1493532684006022No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTTCTGGGGCTGGATCCG18450.1473565669513963No Hit
CAGTAATACACAGCCGAGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGA18430.14719683083545984No Hit
GTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCAGTGGCCCTGTT18010.1438423724007939No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTCAGCAG17940.14328329599501624No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGAAGCAAC17800.1421651431834609No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17370.13873081669082674No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTCAGCAGCTACTTAGCCTGGTA17300.1381717402850491No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17110.1366542471836526No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17050.1361750388358432No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16950.13537635825616082No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16860.13465754573444672No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16330.13042453866213016No Hit
GTTTATTACTGTCAGCAGCGAAGCAACTTCGGCCCTGGGACCAAAGTGGA16140.12890704556073365No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA16030.12802849692308305No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16010.1278687608071466No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15940.12730968440136894No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15750.12579219129997243No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15730.12563245518403598No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15590.12451430237248066No Hit
CTATTATAGTGGGACCACTTACTACAACCCGTCCCTCAAGAGTCGAGTCA15440.1233162815029571No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGAGAACCTGGCTGGGATGCCA15360.12267733703921122No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15070.12036116335813238No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14940.11932287860454528No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14470.11556907988003817No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14450.11540934376410171No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14090.1125340936772452No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13700.10941923941648397No Hit
GGACTGGAGTGGATTGGGAATATCTATTATAGTGGGACCACTTACTACAA13630.1088601630107063No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGAAATCAAAAGGCCCATAAC13350.1066238573875957No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13340.10654398932962744No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13250.10582517680791334No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13230.10566544069197685No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13160.1051063642861992No Hit
CTATAATAGATATTCCCAATCCACTCCAGTCCCTTCCCCGGGGGCTGGCGGATCCAGCCCCAGAAGTAATTACTA13140.10494662817026273No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13060.10430768370651683No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12890.10294992672105681No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAGTTGCTTCGCTGCTGACAGTAATAAACTG12850.10263045448918387No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12680.10127269750372385No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC12580.1004740169240415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)