FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054811
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49050.46501221545850396No Hit
CTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTC48330.45818634807562686No Hit
CTATAATAGTTATTCTGCGGGGTACAGTTTTGGCCAGGGGACCAAACTGG47840.45354096610672434No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43760.4148610509370873No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35200.3337090720517704No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33120.31398989961234763No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT32440.30754324708407477No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29680.28137742211637917No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29280.2775852735703363No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG29220.2770164512884299No Hit
GTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTG28250.26782049106427597No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26370.2499973928978746No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26060.2470584777746914No Hit
GTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA25720.243835151510555No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25540.24212868466483567No Hit
GACTATTAGTAGCTGGTTGGCCTGGTATCAACAGAAATCAGGGAAAGCCCCTAGGCTCCTGATCTATAAGGCGT25520.24193907723753355No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24650.23369115414989036No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24460.23188988359051999No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA24450.23179507987686893No Hit
ATTAGTAGCTGGTTGGCCTGGTATCAACAGAAATCAGGGAAAGCCCCTAG23970.22724450162161752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23900.22658087562606005No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23850.22610685705780467No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAG23790.22553803477589826No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23350.2213666713752511No Hit
GAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTA23250.2204186342387404No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21690.2056292549091733No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21550.20430200291805828No Hit
GGCCAAAACTGTACCCCGCAGAATAACTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG20930.1984241726716919No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20310.19254634242532548No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19570.18553086761514623No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19450.18439322305133338No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19020.18031666336433733No Hit
AAACTGTACCCCGCAGAATAACTATTATAGTGTAGGCAGTAATAAGTTGC18970.17984264479608195No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18510.1754816739681327No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGA18480.17519726282717946No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCA18020.1708362919992302No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17730.16808698430334912No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17710.16789737687604697No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC17480.16571689146207236No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAACAGC17450.16543248032111912No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17180.16287278005254022No Hit
ACTTATTACTGCCTACACTATAATAGTTATTCTGCGGGGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAA17050.1616403317750763No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTG17000.16116631320682093No Hit
CCTTATAGATCAGGAGCCTAGGGGCTTTCCCTGATTTCTGTTGATACCAGGCCAACCAGCTACTAATAGTCTGA16830.15955465007475272No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16800.15927023893379952No Hit
GTTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCACTTTCTAAAGTAGA16750.15879622036554414No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAG16400.15547809038775667No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16310.15462485696489703No Hit
TAGTTATTCTGCGGGGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT16250.1540560346829906No Hit
GATCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCA16200.15358201611473524No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16140.15301319383282883No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16040.15206515669631812No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15740.14922104528678595No Hit
CACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCTACACTATAATAGTTATTCTGCGGGGTA15150.1436276261813728No Hit
GCCTAGGGGCTTTCCCTGATTTCTGTTGATACCAGGCCAACCAGCTACTA15120.14334321504041958No Hit
CCCCTAGGCTCCTGATCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCA15030.1424899816175599No Hit
TCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACT14790.1402146924899342No Hit
TTCTAAAGTAGACGCCTTATAGATCAGGAGCCTAGGGGCTTTCCCTGATT14450.1369913662257978No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14250.13509529195277636No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA13910.13187196568863996No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13590.12883824685180567No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13570.12864863942450352No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13550.12845903199720138No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13280.12589933172862247No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13230.12542531316036712No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13230.12542531316036712No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13190.12504609830576283No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12850.1218227720416264No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12740.12077993119146463No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12610.1195474829140007No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12400.11755660492732821No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12340.1169877826454218No Hit
GAATAACTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTG12330.11689297893177071No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12230.11594494179525999No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12160.11528131579970251No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11950.11329043781303003No Hit
ATAATAGTTATTCTGCGGGGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT11950.11329043781303003No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11880.11262681181747251No Hit
CATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTG11760.11148916725365965No Hit
GCTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA11710.1110151486854043No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11710.1110151486854043No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11700.11092034497175324No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11680.11073073754445109No Hit
TTATAGATCAGGAGCCTAGGGGCTTTCCCTGATTTCTGTTGATACCAGGC11680.11073073754445109No Hit
GGTATCAACAGAAATCAGGGAAAGCCCCTAGGCTCCTGATCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCAT11580.10978270040794037No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11580.10978270040794037No Hit
CACTATAATAGTTATTCTGCGGGGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCA11530.10930868183968503No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11450.10855025213047645No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11280.10693858899840825No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11170.10589574814824647No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11160.10580094443459538No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11160.10580094443459538No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG11120.10542172957999112No Hit
ATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGT11110.10532692586634003No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10820.10257761817045896No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCTTATAGATCAGGAGC10660.10106075875204183No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10640.10087115132473969No Hit
GGGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG10580.10030232904283326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT301.1639895E-768.71834639
CTATCGC253.3236665E-668.173735
GTAAGCT150.002604165367.2753143
TCGTACC252.9089523E-453.8202482
TCGCGTA408.629813E-751.42670438
ACACCGT200.00813334450.4564826
TATCGCG352.4136261E-548.78689636
CTTATAC7800.044.8650931
CGTATGC7350.042.72819541
ACGAGAC8200.042.68667622
TATACAC8300.042.553663
TCGTATG7150.042.3846740
CGTACCC405.807348E-542.047073
ATCTCGT7100.041.92749837
TATCTCG7200.041.73989536
CGAGACG8450.041.43358223
ATGCCGT7500.041.2542844
TTATACA8500.041.1566622
ATCGCGT504.0664836E-641.0600337
CTCTTAT2050.041.035151
Produced by FastQC (version 0.11.5)