FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054811
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72610.6883697648204276No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50320.47705228709219No Hit
CTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTC46610.44188010932764255No Hit
CTATAATAGTTATTCTGCGGGGTACAGTTTTGGCCAGGGGACCAAACTGG45880.4349594382311144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40010.37930965831793567No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34640.32840006408731043No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34630.32830526037365937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28590.2710438173284124No Hit
GTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTG28180.2671568650687185No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27660.26222707195886275No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27610.2617530533906074No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26820.2542635600121728No Hit
GTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA25970.24620524435183172No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA25570.2424130958057889No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG25050.2374833026959332No Hit
GACTATTAGTAGCTGGTTGGCCTGGTATCAACAGAAATCAGGGAAAGCCCCTAGGCTCCTGATCTATAAGGCGT24120.22866655732638358No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23870.22629646448510682No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23800.22563283848954932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23260.22051343795239145No Hit
ATTAGTAGCTGGTTGGCCTGGTATCAACAGAAATCAGGGAAAGCCCCTAG22830.2164368782653954No Hit
GAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTA22450.21283433714665473No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAG22310.2115070851555397No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21980.20837856260505438No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21800.20667209575933507No Hit
GGCCAAAACTGTACCCCGCAGAATAACTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG21360.20250073235868796No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21250.2014578915085262No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20690.19614888354406618No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20560.19491643526660227No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCA20420.19358918327548727No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT20200.19150350157516371No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20130.1908398755796062No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19650.18628929732435479No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19150.18154911164180124No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19060.1806958782189416No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18480.17519726282717946No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18460.1750076553998773No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAACAGC17810.1688454140125577No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17800.16875061029890664No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGA17700.1678025731623959No Hit
AAACTGTACCCCGCAGAATAACTATTATAGTGTAGGCAGTAATAAGTTGC17640.1672337508804895No Hit
GTTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCACTTTCTAAAGTAGA17540.1662857137439788No Hit
GATCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCA17260.16363120976174877No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTG17120.1623039577706338No Hit
ACTTATTACTGCCTACACTATAATAGTTATTCTGCGGGGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAA16850.15974425750205487No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC16770.1589858277928463No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16720.15851180922459093No Hit
CCTTATAGATCAGGAGCCTAGGGGCTTTCCCTGATTTCTGTTGATACCAGGCCAACCAGCTACTAATAGTCTGA16290.15443524953759488No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16150.15310799754647988No Hit
TAGTTATTCTGCGGGGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT15840.15016908242329668No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15780.14960026014139025No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAG15750.14931584900043704No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15540.14732497101376454No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15470.14666134501820705No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15270.1447652707451856No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14780.14011988877628315No Hit
CCCCTAGGCTCCTGATCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCA14720.1395510664943767No Hit
TTCTAAAGTAGACGCCTTATAGATCAGGAGCCTAGGGGCTTTCCCTGATT14400.13651734765754245No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14290.13547450680738066No Hit
TCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACT13920.13196676940229102No Hit
CACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCTACACTATAATAGTTATTCTGCGGGGTA13790.13073432112482708No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13690.1297862839883164No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA13380.1268473688651332No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13360.12665776143783106No Hit
GCCTAGGGGCTTTCCCTGATTTCTGTTGATACCAGGCCAACCAGCTACTA13330.12637335029687782No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13230.12542531316036712No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13170.1248564908784607No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG13060.1238136500282989No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12980.12305522031909033No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12900.12229679060988177No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12730.12068512747781357No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12620.11964228662765176No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12460.11812542720923463No Hit
GAATAACTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTG12210.11575533436795787No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11940.11319563409937894No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11840.11224759696286822No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11840.11224759696286822No Hit
ATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGT11770.11158397096731074No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11680.11073073754445109No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11600.10997230783524252No Hit
CATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTG11490.10892946698508074No Hit
GGTATCAACAGAAATCAGGGAAAGCCCCTAGGCTCCTGATCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCAT11270.10684378528475719No Hit
GCTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA11150.10570614072094432No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11120.10542172957999112No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11000.10428408501617825No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10950.1038100664479229No Hit
TTATAGATCAGGAGCCTAGGGGCTTTCCCTGATTTCTGTTGATACCAGGC10950.1038100664479229No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10900.10333604787966753No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10830.10267242188411005No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10810.1024828144568079No Hit
GGGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG10740.1018191884612504No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10670.10115556246569289No Hit
ATAATAGTTATTCTGCGGGGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT10660.10106075875204183No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10560.10011272161553111No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG10560.10011272161553111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTTA200.007125094552.1741344
GTGGTAT2250.040.3121641
GACAGGT2150.034.3747141
AATGGTA4450.032.38485770
GTGTATA851.5263322E-731.6173861
ACGGGAT652.7666028E-531.03879716
CCGAAAC550.005956146429.1166268
GATTCAC2100.028.7944031
CGAAACC650.00715282128.04487669
CGGGTAC606.2919146E-428.0277917
TCAACGC25400.027.7820324
ATCAACG25600.027.6909943
AACGCAG25550.027.617626
CAACGCA25600.027.563685
AGAGTAC26450.026.4175511
CGCAGAG26750.026.3786968
ACGCAGA26850.026.2804537
GCAGAGT26950.026.1816989
CAGAGTA27050.026.08490810
TATACCT650.001009827525.847265
Produced by FastQC (version 0.11.5)