FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060021
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAACATCGCCGTTACCCTCTCACTTTCGGCGGGGGCACCAGGGTGGAGATCAGACGAACTGTGGCTGCAC34010.3208427002861264No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32710.30857879230694485No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32710.30857879230694485No Hit
GTTGTAGACAGTGATAAGTTGCAAAGTCTTCAGGGTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATC26740.25225915335639576No Hit
GTAATATACGGCCGTGTCGTCAGATCTGAGACTGCTCAGCTCCAGGTAGGCTGTGCTGATGGACTTGTCCCTGGT25210.23782547704243595No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24020.22659928435380053No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22700.21414670086724696No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20600.19433577259318446No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19730.18612838802250145No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19000.17924173200342258No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18960.17886438098867852No Hit
GTAGGAGACAGAGTCACCATTGTTTGCCGGGCAAGTGAGAACATTGCAACTGCTTTAGGCTGGTATCAGCACAAA18550.17499653308755203No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17650.16650613525581096No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17630.16631745974843895No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17550.16556275771895085No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17380.16395901590628867No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16810.15858176394618598No Hit
GTCTTCAGGGTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC16700.15754404865563984No Hit
GCTTTAGGCTGGTATCAGCACAAACCAGGGAAAGCCCCTAAGCGCCTGATTTATGATGGGTCTTTTTTGGAAA16210.15292149872502525No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16120.15207245894185117No Hit
CCCTGAAGACTTTGCAACTTATCACTGTCTACAACATCGCCGTTACCCTC16030.15122341915867704No Hit
AGTGAGAACATTGCAACTGCTTTAGGCTGGTATCAGCACAAACCAGGGAA15320.1445254386469702No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15060.1420726570511339No Hit
GTATATTACTGTGTGAGATCGATGTGGGAACTAAGTTCTCCTGACTACTGGGGCCAGGGAACTCTGGTCACCGT14990.1414122927753318No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATTGTTTGCCGGGCAAGTGAGAACATTGCAA14990.1414122927753318No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14880.14037457748478568No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14870.14028023973109968No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT14600.13773312038157734No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13430.12669560320031395No Hit
ACTTATCACTGTCTACAACATCGCCGTTACCCTCTCACTTTCGGCGGGGGCACCAGGGTGGAGATCAGACGAA13300.1254692124023958No Hit
CTCTAATACTGGTGACACAAACTTTGCACAGAAGTTTCAGGGCAGGGTCACCATGACCAGGGACAAGTCCATCAG13210.1246201726192217No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13180.12433715935816365No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13100.12358245732867557No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13010.12273341754550145No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12960.1222617287770714No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12920.12188437776232736No Hit
GTATTAGAGATGATCCATCCCATCCACTCAAGCCCTTGCCCAGGGGCCTGTCGCACCCAATGTATGTAATAACTG12840.12112967573283927No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12830.12103533797915324No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12470.1176391788464568No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12380.11679013906328271No Hit
GTGATAAGTTGCAAAGTCTTCAGGGTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12130.11443169522113242No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCCTGGTGC11980.11301662891584224No Hit
CAGTAATATACGGCCGTGTCGTCAGATCTGAGACTGCTCAGCTCCAGGTA11880.11207325137898212No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11660.10999782079788986No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11380.10735636369468152No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11340.10697901267993748No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11090.10462056883778717No Hit
GAATTCACTCTCACAATCAGCAGCCTGCACCCTGAAGACTTTGCAACTTATCACTGTCTACAACATCGCCGTTAC10820.10207344948826484No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10820.10207344948826484No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10790.10179043622720682No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10610.10009235666085861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)