FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060021
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAACATCGCCGTTACCCTCTCACTTTCGGCGGGGGCACCAGGGTGGAGATCAGACGAACTGTGGCTGCAC33670.31763521666080197No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32350.30518263317424843No Hit
GTTGTAGACAGTGATAAGTTGCAAAGTCTTCAGGGTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATC30170.28461700287069785No Hit
GTAATATACGGCCGTGTCGTCAGATCTGAGACTGCTCAGCTCCAGGTAGGCTGTGCTGATGGACTTGTCCCTGGT26790.2527308421248258No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25210.23782547704243595No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24560.23169352305284519No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22080.20829776013871426No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20650.19480746136161453No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19750.18631706352987346No Hit
GTCTTCAGGGTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC18660.17603424837809817No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17250.1627326251083705No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16670.1572610353945818No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16630.15688368437983777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16440.15509126705980356No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16250.1532988497397693No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16230.15311017423239728No Hit
GCTTTAGGCTGGTATCAGCACAAACCAGGGAAAGCCCCTAAGCGCCTGATTTATGATGGGTCTTTTTTGGAAA15540.14660086922806248No Hit
CCCTGAAGACTTTGCAACTTATCACTGTCTACAACATCGCCGTTACCCTC15290.14424242538591217No Hit
GTATATTACTGTGTGAGATCGATGTGGGAACTAAGTTCTCCTGACTACTGGGGCCAGGGAACTCTGGTCACCGT15270.14405374987854014No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15230.1436763988637961No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14970.1412236172679598No Hit
GTAGGAGACAGAGTCACCATTGTTTGCCGGGCAAGTGAGAACATTGCAACTGCTTTAGGCTGGTATCAGCACAAA14700.13867649791843745No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14330.13518600103205503No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14320.13509166327836902No Hit
GTGATAAGTTGCAAAGTCTTCAGGGTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14040.13245020617516068No Hit
CTCTAATACTGGTGACACAAACTTTGCACAGAAGTTTCAGGGCAGGGTCACCATGACCAGGGACAAGTCCATCAG13800.1301861000866964No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13780.12999742457932437No Hit
GTATTAGAGATGATCCATCCCATCCACTCAAGCCCTTGCCCAGGGGCCTGTCGCACCCAATGTATGTAATAACTG13740.12962007356458033No Hit
AGTGAGAACATTGCAACTGCTTTAGGCTGGTATCAGCACAAACCAGGGAA13730.1295257358108943No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13660.12886537153509223No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATTGTTTGCCGGGCAAGTGAGAACATTGCAA13580.12811066950560412No Hit
ACTTATCACTGTCTACAACATCGCCGTTACCCTCTCACTTTCGGCGGGGGCACCAGGGTGGAGATCAGACGAA13080.12339378182130353No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12520.11811086761488689No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12170.11480904623587646No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12150.11462037072850442No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11900.11226192688635413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11830.11160156261055204No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11600.10943179427577378No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11410.10763937695573957No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11260.10622431065044939No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11060.10433755557672914No Hit
CAGTAATATACGGCCGTGTCGTCAGATCTGAGACTGCTCAGCTCCAGGTA10950.10329984028618301No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT10850.10235646274932289No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10690.1008470586903467No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10640.10037536992191663No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCCTGGTGC10640.10037536992191663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)