FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782838
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGGACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT76150.4271279835857212No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC67810.3803486351536146No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA59010.33098913081278275No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTTACCATCTCCTGCACCCGCAGTAGTGGCAACATTGCCAA55080.308945624896934No Hit
GTAATATAGAGCCGAGTCTTCAGGTCTCAGGTTGTTCATTTCCAAGTACA48730.2733282552873565No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTTACCATCTCCTGCACCCGCAGTAGTGGCAACATTGCCAACAACTATGTG46390.26020311436036253No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45450.25493062185122817No Hit
CTATATTACTGTACGAGAGATGCGGGTGGAGGATATGATGGTAGTGATTG44560.24993858107130318No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44020.24690970239584303No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCGCCGCCGAAAATGGCAGGATTTTTACTATCATAAGACTGA38280.2147138438826186No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36720.20596374993128932No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCGC34840.1954187649130207No Hit
GACTACTACTGTCAGTCTTATGATAGTAAAAATCCTGCCATTTTCGGCGGCGGGACCAAGCTGACCGTCCTGGGT31800.17836729977709695No Hit
CTCCAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGCAGAGCATAGTCAC31100.17444097556816718No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28920.1622132801746429No Hit
CTGCACAGGTTCTTGGGCCAATTTTATTCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTTAC28660.1607549311827547No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28130.1577821428531364No Hit
ATATTACTGTACGAGAGATGCGGGTGGAGGATATGATGGTAGTGATTGGTTCTACTTACACTTCCAGCACTGGGG27830.15609943247788077No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27650.1550898062527274No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGAGG27600.15480935452351813No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27000.15144393377300686No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26420.1481906937141793No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26330.14768588060160262No Hit
GTCTTATGATAGTAAAAATCCTGCCATTTTCGGCGGCGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGC26300.14751760956407703No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26180.1468445254139748No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25990.14577880884297956No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25820.14482527296366804No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25710.14420827915940765No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAG25630.1437595563926728No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25260.1416842135965242No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25180.14123549082978934No Hit
ATTTTATTCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG25040.14045022598800339No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24930.139833232183743No Hit
CTATGAGGGTAACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGC24620.13809443146264552No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGA24440.1370848052374921No Hit
CTCCTGTGCAGCCTCCGGATTCAGGTTTAGTGACTATGCTCTGCACTGGGTCCGCCAGGCTCCAGGCAAGGGC24300.13629954039570616No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24140.1354020948622365No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23720.13304630033687861No Hit
GGCCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGCAATAAATTCTA23120.12968087958636737No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT22930.12861516301537212No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22930.12861516301537212No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22920.1285590726695303No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22740.12754944644437688No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22120.12407184500218191No Hit
CTACTACTGTCAGTCTTATGATAGTAAAAATCCTGCCATTTTCGGCGGCG21880.1227256767019774No Hit
CAGTAATATAGAGCCGAGTCTTCAGGTCTCAGGTTGTTCATTTCCAAGTA21560.12093078563503808No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21220.11902371387641501No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21200.1189115331847313No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20770.11649964831353157No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20610.1156022027800619No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA20520.11509738966748523No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20340.11408776344233183No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT20220.11341467929222959No Hit
CAACTATGTGCAGTGGTTCCAGCAGCGCCCGGGCAGTTCCCCCACCAATGTGATCTATGAGGGTAACCAAAGACC19500.10937617439161607No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT18760.10522548879931884No Hit
CCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCAT18670.10472067568674216No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18670.10472067568674216No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATTCTGA18650.10460849499505845No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18600.10432804326584917No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA18490.10371104946158877No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18460.1035427784240632No Hit
CCCTCATAGATCACATTGGTGGGGGAACTGCCCGGGCGCTGCTGGAACCACTGCACATAGTTGTTGGCAATGTTG18400.10320623634901209No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18180.1019722487404913No Hit
CTCCTGCACCCGCAGTAGTGGCAACATTGCCAACAACTATGTGCAGTGGTTCCAGCAGCGCCCGGGCAGTTCCCC18150.10180397770296573No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18070.10135525493623089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3900.051.2801970
GTGGTAT3700.049.9572941
CGTATGC3450.049.02776341
ATGCCGT3300.047.2150444
ATCTCGT3000.047.0263837
TCGTATG3650.045.35964240
GTATGCC3700.043.88889342
CACGAGA3900.042.99727221
CGAGACG3850.041.78023523
TGGTATC4450.041.5258752
GGTATCA13250.041.3343351
GCCGTCT3650.040.86223246
TCTCCGA4150.039.58021511
TATACAC4500.039.543573
CTTATAC4100.039.2392651
ACGAGAC4300.038.99752422
CTACATC3750.038.4465333
CCCACGA4400.038.11014619
TTATACA4400.038.109082
CATCTCG3800.037.10306536
Produced by FastQC (version 0.11.5)