FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782838
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGGACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT69190.38808910287979054No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61990.3477040538736554No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCGCCGCCGAAAATGGCAGGATTTTTACTATCATAAGACTGA61620.34562871107750676No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA60120.3372151592012286No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47930.2688410276200081No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTTACCATCTCCTGCACCCGCAGTAGTGGCAACATTGCCAA47870.268504485544957No Hit
GTAATATAGAGCCGAGTCTTCAGGTCTCAGGTTGTTCATTTCCAAGTACA45850.2571742356849024No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTTACCATCTCCTGCACCCGCAGTAGTGGCAACATTGCCAACAACTATGTG43930.24640488928326637No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42280.23714998221936034No Hit
CTATATTACTGTACGAGAGATGCGGGTGGAGGATATGATGGTAGTGATTG41510.23283102558953758No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34260.19216552485419314No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33440.18756611649516108No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCGC32540.18251798536939418No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31890.17887211288967364No Hit
CTCCAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGCAGAGCATAGTCAC31370.17595541490589722No Hit
GACTACTACTGTCAGTCTTATGATAGTAAAAATCCTGCCATTTTCGGCGGCGGGACCAAGCTGACCGTCCTGGGT30620.17174863896775816No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28220.1582869559657131No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27900.15649206489877374No Hit
CTGCACAGGTTCTTGGGCCAATTTTATTCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTTAC27690.1553141676360948No Hit
GTCTTATGATAGTAAAAATCCTGCCATTTTCGGCGGCGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGC27350.15340709587747176No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27290.15307055380242063No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGAGG27230.15273401172736953No Hit
ATATTACTGTACGAGAGATGCGGGTGGAGGATATGATGGTAGTGATTGGTTCTACTTACACTTCCAGCACTGGGG26020.14594707988050512No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25960.145610537805454No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25880.14516181503871917No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAG25770.14454482123445878No Hit
CTCCTGTGCAGCCTCCGGATTCAGGTTTAGTGACTATGCTCTGCACTGGGTCCGCCAGGCTCCAGGCAAGGGC24860.13944059976285003No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24270.1361312693581806No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24250.1360190886664969No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24080.13506555278718538No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23860.13383156517866457No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23510.13186840307419967No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23270.13052223477399516No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGA23240.1303539637364696No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22730.12749335609853504No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22620.12687636229427465No Hit
ATTTTATTCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG22570.12659591056506536No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22080.12384748361881451No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22050.12367921258128894No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21970.1232304898145541No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21950.12311830912287039No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21680.12160386978514032No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21470.12042597252246137No Hit
GGCCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGCAATAAATTCTA21370.11986506906404285No Hit
CTATGAGGGTAACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGC20970.11762145523036865No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA20300.1138634020589644No Hit
CAGTAATATAGAGCCGAGTCTTCAGGTCTCAGGTTGTTCATTTCCAAGTA20260.11363904067559699No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA19810.11111497511271354No Hit
GACCCAGGACGGTCAGCTTGGTCCCGCCGCCGAAAATGGCAGGATTTTTACTATCATAAGACTGACAGTAGTAGT19670.11032971027092758No Hit
CCCTCATAGATCACATTGGTGGGGGAACTGCCCGGGCGCTGCTGGAACCACTGCACATAGTTGTTGGCAATGTTG19630.11010534888756017No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19510.1094322647374579No Hit
CTACTACTGTCAGTCTTATGATAGTAAAAATCCTGCCATTTTCGGCGGCG19150.10741301228715115No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18790.1053937598368444No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18480.10365495911574692No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC18190.10202833908633313No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC17890.10034562871107751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.043.2495351
GTGGTAT4150.041.1771931
TGGTATC4300.038.1532942
TCTGGAG21200.037.7948170
GTATCAA35550.036.4363171
ATCGTAC400.00256495934.5866643
CAGAGGG29350.032.4185570
AATGGTA6850.032.37584770
ATCAACG40700.031.7454153
TATCAAC40700.031.6596552
TCAACGC41100.031.350654
GGAACGA450.00460898930.68618241
AACGCAG42100.030.6854426
CAACGCA42100.030.5239435
GTGTTAC802.9728672E-629.904941
GACAGCC3000.029.620131
TTTTCGG34150.029.32833370
CGCAGAG45200.028.6565198
AGAGTAC45500.028.16159611
CAGAGTA46250.027.561810
Produced by FastQC (version 0.11.5)