FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010141
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGGACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT65330.6467413955081518No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAACCATGTCCTGCACCCGCAGCAGTGGCAGCATTGCCAA45510.4505311634712382No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA44070.43627572784393465No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAACTCG37390.3701463459061656No Hit
GTGTCGGAGTCTCCGGGGAAGACAGTAACCATGTCCTGCACCCGCAGCAGTGGCAGCATTGCCAACAACTACGTG35710.35351500434097816No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34830.3448033492354038No Hit
TAATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGA32890.3255981095708421No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32830.3250041330863711No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27830.2755060927137895No Hit
GTCTTATTATGATCACAATCGAGTTTTCGGCGGGGGGACCAAGCTGACCGTCCTGAGTCAGCCCAAGGCTGCCCC26470.26204262573244724No Hit
GTAATATACGGCCGTGTCGTCAGATGTCAGTCTGCTCAGCTCCATGTAGGCTGTACTGATGGACGTGTCCCTGGT25810.25550888440326647No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCCC25610.25352896278836323No Hit
GACTACTACTGTCAGTCTTATTATGATCACAATCGAGTTTTCGGCGGGGGGACCAAGCTGACCGTCCTGAGTCAG24740.24491630376353402No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23720.23481870352752734No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAAC23050.2281859661176014No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22720.22491909545301103No Hit
ACCATGTCCTGCACCCGCAGCAGTGGCAGCATTGCCAACAACTACGTGCA21600.21383153440955271No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21250.21036667158347203No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21100.20888173037229454No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGAGG21070.2085847421300591No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21070.2085847421300591No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21020.20808976172633326No Hit
GGTACCAACAGCGCCCGGGCAGTTCCCCCACCATTGTGATCTATGAGAAT20380.20175401255864278No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19940.19739818500585563No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACA19900.19700220068287494No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19670.19472529082573622No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19540.1934383417760491No Hit
GTATATTACTGTGCGAGAGAAAGTTATCACTGGTATTACGACACCTGGGGCCAGGGAACCCTGGTCACCGTCTC18740.18551865531643602No Hit
CCCCTGGACAGGGGCTTGAGTGGTTGGGATGGATCAACCCTGACACTGAT18590.18403371410525857No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18130.17947989439098105No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17690.17512406683819387No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17650.17472808251521324No Hit
GTGGTACCAACAGCGCCCGGGCAGTTCCCCCACCATTGTGATCTATGAGAATAGCCAAAGACCCTCTGGGGTCC17490.1731441452232906No Hit
CTACTACTGTCAGTCTTATTATGATCACAATCGAGTTTTCGGCGGGGGGACCAAGCTGACCGTCCTGAGTCAGCC17100.16928329807422926No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT16680.16512546268293238No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAA16200.16037365080716454No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16100.15938368999971292No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16090.15928469391896774No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGA15820.15661179973884834No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT15800.156413807577358No Hit
GTACCAACAGCGCCCGGGCAGTTCCCCCACCATTGTGATCTATGAGAATA15610.15453288204319993No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15370.15215697610531598No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15270.15116701529786436No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15190.15037504665190304No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA14980.1482961289562546No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14980.1482961289562546No Hit
GCCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC14610.14463327396868356No Hit
CAGTAACCATGTCCTGCACCCGCAGCAGTGGCAGCATTGCCAACAACTAC14500.1435443170804868No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14470.1432473288382513No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14210.14067343073887706No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA13590.13453567373267694No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13590.13453567373267694No Hit
GTCCTGCACCCGCAGCAGTGGCAGCATTGCCAACAACTACGTGCAGTGGTACCAACAGCGCCCGGGCAGTTCCCC13530.13394169724820595No Hit
GTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAGGACTGAGGACGAGG13510.1337437050867156No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13270.13136779914883168No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12840.12711096767678967No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG12680.12552703038486707No Hit
GACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAACTCGATTGTGATCATAATAAGACTGACAGTAGTAGTCAG12670.1254280343041219No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12610.12483405781965093No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12450.1232501205277283No Hit
GCCAACAACTACGTGCAGTGGTACCAACAGCGCCCGGGCAGTTCCCCCAC12210.12087421458984438No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACT12170.12047823026686373No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAACTCGATTGTGAT12080.11958726554015726No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12080.11958726554015726No Hit
ACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATTATGATCACAATCGAGTTTTCGGCGGGGGGAC12060.11938927337866695No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12040.1191912812171766No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11990.11869630081345078No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11910.1179043321674895No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11890.11770634000599915No Hit
CCCTGACACTGATGCCACAAATTATGCACAGAAGTTTCAGGGCAGGGTCA11870.11750834784450884No Hit
ACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAACTCGATTGTGATCATAATAAGACTGACAGTAGTAGTCAGC11740.11622139879482173No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC11700.11582541447184107No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT11700.11582541447184107No Hit
CCCCAGAGGGTCTTTGGCTATTCTCATAGATCACAATGGTGGGGGAACTGCCCGGGCGCTGTTGGTACCACTGCA11490.11374649677619263No Hit
CAACTACGTGCAGTGGTACCAACAGCGCCCGGGCAGTTCCCCCACCATTGTGATCTATGAGAATAGCCAAAGACC11460.11344950853395715No Hit
CCATTGTGATCTATGAGAATAGCCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCT11380.11265753988799583No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11380.11265753988799583No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11320.11206356340352486No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11220.11107360259607323No Hit
GTTGGTACCACTGCACGTAGTTGTTGGCAATGCTGCCACTGCTGCGGGTG11050.10939066922340546No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACT10950.10840070841595381No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10950.10840070841595381No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAACCATGTC10760.10651978288179571No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10540.10434186910540212No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10480.10374789262093113No Hit
GTCCTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACC10430.10325291221720533No Hit
GTCTTTGGCTATTCTCATAGATCACAATGGTGGGGGAACTGCCCGGGCGC10400.10295592397496983No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG10310.10206495924826336No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC10290.10186696708677304No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10190.10087700627932139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6650.045.8793071
ATCTCGT1000.041.28193337
TCGTATG1200.040.21318440
CTTATAC1550.039.8097341
CGTATGC1300.039.8010241
GCGGAGT350.001396439539.1448634
GTATCAA16500.039.059111
TTTGCGC451.0446764E-438.05939517
ATTTGCG451.0446764E-438.05939516
TGTCTTG750.001669007437.7459570
TGGTATC1750.037.187622
ATGCCGT1400.037.0800944
ACGGGTA655.765287E-736.88833216
GTGGTAT1800.036.1850851
GATCTCG1151.8189894E-1235.88484636
GTATGCC1450.035.72106642
TCTCCGA1550.035.35665511
ACGAGAC1750.035.23212422
GTACAGA400.002678681634.2806051
ACAGCGG601.4023299E-534.2806051
Produced by FastQC (version 0.11.5)