FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010141
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGGACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT60330.5972433551355701No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA47620.4714193365084676No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAACTCG37800.3742051852167173No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAACCATGTCCTGCACCCGCAGCAGTGGCAGCATTGCCAA37110.367374455645301No Hit
GTGTCGGAGTCTCCGGGGAAGACAGTAACCATGTCCTGCACCCGCAGCAGTGGCAGCATTGCCAACAACTACGTG35270.349159176788191No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33760.3342107685956713No Hit
TAATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGA33190.328567991993197No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33190.328567991993197No Hit
GTAATATACGGCCGTGTCGTCAGATGTCAGTCTGCTCAGCTCCATGTAGGCTGTACTGATGGACGTGTCCCTGGT30580.3027300149187094No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26640.263725559105115No Hit
GTCTTATTATGATCACAATCGAGTTTTCGGCGGGGGGACCAAGCTGACCGTCCTGAGTCAGCCCAAGGCTGCCCC25780.255211896161031No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCCC24730.24481730768278884No Hit
GACTACTACTGTCAGTCTTATTATGATCACAATCGAGTTTTCGGCGGGGGGACCAAGCTGACCGTCCTGAGTCAG24190.23947151932255No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22930.22699801314865947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22640.22412712680704971No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAA22520.22293917383810774No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAAC22180.2195733070927722No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22150.2192763188505367No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGAGG21940.21719740115488825No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21280.21066365982570748No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20550.20343694593131056No Hit
ACCATGTCCTGCACCCGCAGCAGTGGCAGCATTGCCAACAACTACGTGCA20440.2023479890431138No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20330.201259032154917No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20290.20086304783193631No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20270.20066505567044599No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19800.19601223987542335No Hit
GTGGTACCAACAGCGCCCGGGCAGTTCCCCCACCATTGTGATCTATGAGAATAGCCAAAGACCCTCTGGGGTCC19760.1956162555524427No Hit
GTATATTACTGTGCGAGAGAAAGTTATCACTGGTATTACGACACCTGGGGCCAGGGAACCCTGGTCACCGTCTC18690.1850236749127102No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18570.18383572194376827No Hit
GGTACCAACAGCGCCCGGGCAGTTCCCCCACCATTGTGATCTATGAGAAT18280.1809648356021585No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17680.17502507075744872No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACA17300.1712632196891325No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17160.16987727455870022No Hit
CTACTACTGTCAGTCTTATTATGATCACAATCGAGTTTTCGGCGGGGGGACCAAGCTGACCGTCCTGAGTCAGCC16790.16621441957112917No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16730.16562044308665821No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGA16560.16393750971399043No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16020.1585917213537516No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15930.15770075662704514No Hit
CCCCTGGACAGGGGCTTGAGTGGTTGGGATGGATCAACCCTGACACTGAT15620.15463187812394508No Hit
GTTGGTACCACTGCACGTAGTTGTTGGCAATGCTGCCACTGCTGCGGGTG15020.1486921132792353No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14780.14631620734135137No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA14710.1456232347761352No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14450.14304933667676098No Hit
GTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAGGACTGAGGACGAGG14220.1407724268196222No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14010.13869350912397377No Hit
GTCTTTGGCTATTCTCATAGATCACAATGGTGGGGGAACTGCCCGGGCGC13960.138198528720248No Hit
GTACCAACAGCGCCCGGGCAGTTCCCCCACCATTGTGATCTATGAGAATA13790.13651559534758018No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13740.13602061494385437No Hit
GTCCTGCACCCGCAGCAGTGGCAGCATTGCCAACAACTACGTGCAGTGGTACCAACAGCGCCCGGGCAGTTCCCC13410.13275374427926398No Hit
GACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAACTCGATTGTGATCATAATAAGACTGACAGTAGTAGTCAG13360.13225876387553817No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13140.13008085009914458No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13010.12879390104945745No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12950.12819992456498647No Hit
GCCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC12780.12651699119231868No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12750.1262200029500832No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12730.12602201078859288No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12310.12186417539729602No Hit
CAGTAACCATGTCCTGCACCCGCAGCAGTGGCAGCATTGCCAACAACTAC12310.12186417539729602No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAACTCGATTGTGAT12190.12067622242835406No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12160.12037923418611858No Hit
ACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAACTCGATTGTGATCATAATAAGACTGACAGTAGTAGTCAGC12100.1197852577016476No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12090.11968626162090243No Hit
ACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATTATGATCACAATCGAGTTTTCGGCGGGGGGAC12020.11899328905568629No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12000.11879529689419596No Hit
GTCCTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACC11990.11869630081345078No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG11870.11750834784450884No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11820.11701336744078303No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11600.11483545366438944No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAACCATGTC11360.11245954772650552No Hit
CCCCAGAGGGTCTTTGGCTATTCTCATAGATCACAATGGTGGGGGAACTGCCCGGGCGCTGTTGGTACCACTGCA11330.11216255948427002No Hit
CCCTGACACTGATGCCACAAATTATGCACAGAAGTTTCAGGGCAGGGTCA11150.11038063003085707No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAGCTTGGTCCCCCCGCCGAAAACT11030.10919267706191511No Hit
CTGTTGGTACCACTGCACGTAGTTGTTGGCAATGCTGCCACTGCTGCGGGTGCAGGACATGGTTACTGTCTTCCC11010.10899468490042478No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10990.10879669273893447No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10970.10859870057744413No Hit
GCCAACAACTACGTGCAGTGGTACCAACAGCGCCCGGGCAGTTCCCCCAC10890.10780673193148282No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10890.10780673193148282No Hit
CCATTGTGATCTATGAGAATAGCCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCT10720.10612379855881507No Hit
GTCCAGGGGCCTGTCGCACCCAGTGAATATAGTAGGCGCTGAAGGTGTATCCAGAAGTCTTGCAGGAGACCCTCA10680.10572781423583441No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT10610.10503484167061826No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10530.10424287302465696No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10480.10374789262093113No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC10400.10295592397496983No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC10280.10176797100602787No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10120.10018403371410527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)