FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367325
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45340.33159636516556046No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT44950.328744080595323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41210.3013914029217633No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32550.23805605836213045No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31580.23096191468743713No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29010.21216609072459No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27720.2027316109922659No Hit
GGTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGGCG27700.20258533998866401No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27500.20112262995264477No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCGCCAGCTATTTAAATTGGTATCAGCAGAAA25370.1855447680690399No Hit
CCCCTAACCTCCTCATACTTGCTGCGTCCAGTTTGCAAGCTGGGGTCCCA24910.18218053498619569No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24890.18203426398259373No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT24700.18064468944837547No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24160.17669537235112354No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24140.17654910134752164No Hit
GACTTCACTCTCACCATCACCAATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACCGAGCC24120.17640283034391968No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23780.173916223282687No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCGCCAG23520.172014700235862No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23080.16879673815661966No Hit
GAGTTACCGAGCCCCATTCAGTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT23010.16828478964401294No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22710.16609072458998408No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22700.16601758908818312No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22640.16557877607737737No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22050.1612637814711206No Hit
TACTTGCTGCGTCCAGTTTGCAAGCTGGGGTCCCATCAAGGTTCGGAGGCAGTGGAATTGGGACAGACTTCACT21400.1565099738540581No Hit
GAGTGAAGTCTGTCCCAATTCCACTGCCTCCGAACCTTGATGGGACCCCA20890.15278006326220905No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20870.15263379225860713No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20580.15051286270637926No Hit
AGTCAGAGCATCGCCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAGGCCCCTAACCTCCTCATACTTG20290.14839193315415136No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCC19950.14590532609291865No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT19790.14473515806410325No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19730.1442963450532975No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAACTG19240.14071270546505038No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19100.13968880843983691No Hit
ATAATAGACGGCCGTGTCTTCGGCGCTCAGACCGTTCATTTGTAAAAATA19100.13968880843983691No Hit
GAATTGGGACAGACTTCACTCTCACCATCACCAATCTGCAACCTGAAGAT18780.13734847238220613No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18230.13332601978315323No Hit
ACTTACTACTGTCAACAGAGTTACCGAGCCCCATTCAGTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAA18200.13310661327775036No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17710.12952297368950325No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17650.12908416067869746No Hit
CCTCATACTTGCTGCGTCCAGTTTGCAAGCTGGGGTCCCATCAAGGTTCGGAGGCAGTGGAATTGGGACAGACTT17590.1286453476678917No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17150.12542738558864935No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC17060.12476916607244072No Hit
CGGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATTGGTGATGGTGAGAGTGAAGTCTGTC16920.12374526904722724No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16840.12316018503281956No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16670.12191688150220321No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG16610.12147806849139745No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16450.12030790046258205No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16270.11899146143016473No Hit
CAATAATAGACGGCCGTGTCTTCGGCGCTCAGACCGTTCATTTGTAAAAA16260.11891832592836377No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16240.11877205492476185No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16080.11760188689594647No Hit
CACCATCACCAATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACCGAGCCCCATTCAGTTT16050.1173824803905436No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15830.11577349935092242No Hit
GTATTAGTGGTGGTGGTCATAACACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT15800.11555409284551954No Hit
CAGCAAGTATGAGGAGGTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGGCGATGCTCTGA15700.11482273782750992No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGATTGGTGATGGTGAGAGTGA15600.1140913828095003No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15590.11401824730769934No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15290.11182418225367048No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15220.11131223374106376No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15110.11050774322125317No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA14940.10926443969063683No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC14690.10743605214561278No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14520.10619274861499643No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13970.10217029601594355No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13850.10129266999433201No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13720.10034190847091949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTT200.00810249550.5058222
CCGACGC350.001238575640.11272448
CTTATAC10600.039.700021
ATCTCGT9350.039.1800537
CTTGAAA10450.038.552957
CATCTCG9550.038.2755136
CGTATGC10650.038.256841
TCGTATG10600.038.03160540
TTATACA11150.038.0269622
CCGTCTT10600.037.99899347
TCTACAT10300.037.8928132
TATACAC11200.037.85723
GTGGTAT2850.037.799991
CTCTTAT3300.037.7462961
GTATCAA22500.037.555871
ATGCCGT11050.037.46557244
TACATCT9850.037.33046734
ACGAGAC11250.037.11242322
GACAGGT2100.036.871871
GTATGCC11300.036.75972742
Produced by FastQC (version 0.11.5)