FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367325
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104350.7631689612930357No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46970.34351745195911726No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34950.2556085787943613No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32990.24127402044137278No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32050.23439928327208234No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29200.21355566525880823No Hit
GGTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGGCG27410.20046441043643612No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26870.19651509333918418No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26120.19102993070411203No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCGCCAGCTATTTAAATTGGTATCAGCAGAAA24680.18049841844477355No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT24430.1786700308997495No Hit
GAGTGAAGTCTGTCCCAATTCCACTGCCTCCGAACCTTGATGGGACCCCA23900.17479384930429853No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23560.17230724224306584No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCGCCAG23110.16901614466202255No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22800.16674894410619276No Hit
CCCCTAACCTCCTCATACTTGCTGCGTCCAGTTTGCAAGCTGGGGTCCCA22730.166236995593586No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22420.16396979503775622No Hit
GACTTCACTCTCACCATCACCAATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACCGAGCC21720.1588503099116889No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21660.1584114969008831No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21200.15504726381803888No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21140.1546084508072331No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC21140.1546084508072331No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20900.15285319876401002No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20780.15197557274239848No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20720.1515367597315927No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20200.14773371363794272No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19990.1461978681001225No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19710.14415007404969557No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAACTG19390.1418097379920648No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCC19100.13968880843983691No Hit
TACTTGCTGCGTCCAGTTTGCAAGCTGGGGTCCCATCAAGGTTCGGAGGCAGTGGAATTGGGACAGACTTCACT19000.13895745342182728No Hit
AGTCAGAGCATCGCCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAGGCCCCTAACCTCCTCATACTTG18910.13829923390561863No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18690.13669025286599748No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18420.1347155943173715No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18280.13369169729215805No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18280.13369169729215805No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17470.12776772164628014No Hit
CGGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATTGGTGATGGTGAGAGTGAAGTCTGTC16810.12294077852741667No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16740.12242883001480993No Hit
CCTCATACTTGCTGCGTCCAGTTTGCAAGCTGGGGTCCCATCAAGGTTCGGAGGCAGTGGAATTGGGACAGACTT16550.12103925548059168No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16430.12016162945898012No Hit
ATAATAGACGGCCGTGTCTTCGGCGCTCAGACCGTTCATTTGTAAAAATA16230.1186989194229609No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16220.11862578392115994No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16220.11862578392115994No Hit
GAATTGGGACAGACTTCACTCTCACCATCACCAATCTGCAACCTGAAGAT16090.11767502239774742No Hit
GTATTAGTGGTGGTGGTCATAACACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT16090.11767502239774742No Hit
CAATAATAGACGGCCGTGTCTTCGGCGCTCAGACCGTTCATTTGTAAAAA15730.1150421443329128No Hit
CAGCAAGTATGAGGAGGTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGGCGATGCTCTGA15560.11379884080229645No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15330.11211672426087434No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15310.11197045325727242No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGATTGGTGATGGTGAGAGTGA15240.11145850474466568No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14950.1093375751924378No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14930.10919130418883585No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14900.10897189768343298No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14850.10860622017442817No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA14710.10758232314921472No Hit
CACCATCACCAATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACCGAGCCCCATTCAGTTT14600.10677783262940412No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14410.10538825809518586No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14340.10487630958257912No Hit
CTCCTGTGCAACCTCTGGATTCACCTTTGACAACTATGCCATGACCTGGGTCCGCCAGGGTCCAGGGAAGGGG14100.10312105753935603No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCGCCGTCTCCTCGGCATCCCCGACCAGCCCCAAGGTCTTCCCG13820.10107326348892912No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13750.10056131497632238No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13750.10056131497632238No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13750.10056131497632238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21350.035.391241
GACAGGT2500.033.582351
ATTAGGT651.7333869E-533.23641657
GTGGTAT3300.032.5647051
TACTCAT900.00350644932.4457970
AAGTGAT13600.030.59685370
CACGCTT450.005187293529.95288827
TATCAAC24900.029.8027552
ATCAACG25900.028.780663
TATTAGG754.7038673E-528.71703556
AACGCAG26450.028.6931326
TCAACGC26100.028.5601184
CAACGCA26350.028.2901865
ATAGCGA500.006928181728.23019250
TCTGGAG10400.028.07808570
AGAGTAC27550.027.66533511
GCTTGTG1354.2018655E-1027.3633961
CGCAGAG27900.027.3222778
GCAGAGT28350.026.888599
TATGATA550.00899485626.75936963
Produced by FastQC (version 0.11.5)