FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004689527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602267
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG35750.5935905503705167No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT35100.5827979949092346No Hit
GTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG33250.5520807216732778No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24860.41277373656534394No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGTCATCCCATG22710.3770752838857185No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC19220.3191275630243729No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18650.3096633220814024No Hit
CTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCG16790.2787800095306567No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG16540.27462902666093275No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15620.2593534097003488No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15560.25835717381161516No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15120.25105144396090107No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC14770.24524006794328762No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCT14410.2392626526108852No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13910.23096068687143745No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13780.22880217577918102No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13000.21585110922564243No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12510.2077151828009836No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12090.20074153157984748No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12000.19924717774674688No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11460.19028105474814325No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11460.19028105474814325No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11080.18397156078616295No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10930.18148097106432862No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10500.1743412805284035No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10430.17317900532488084No Hit
GCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG10360.17201673012135812No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9930.16487703958543304No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9920.1647110002706441No Hit
CAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA9720.16139021397486497No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGC9490.15757130973471897No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC9370.15557883795725153No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9300.15441656275372884No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9100.15109577645794972No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8700.1444542038663915No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA8520.14146549620019028No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8500.14113341757061237No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8370.13897490647835595No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8190.13598619881215473No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8130.134989962923421No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC8010.13299749114595355No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC7900.13117105868327503No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7860.1305069014241192No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG7710.12801631170228486No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7700.1278502723874959No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG7570.12569176129523948No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGACTATTACTGTGCAG7540.12519364335087263No Hit
GTCAAGGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGAC7290.12104266048114873No Hit
ATCCCATGCTGCACAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCAC7160.1188841493888923No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7100.11788791350015858No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7080.11755583487058066No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6970.11572940240790214No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG6910.1147331665191684No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6900.11456712720437946No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC6870.1140690092600126No Hit
CCCAACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGG6840.11357089131564571No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6780.11257465542691199No Hit
CCCCTGAGGGCCCTTGACTATTCTTATAAATGAGGAGCTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTATA6740.11191049816775617No Hit
CTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC6620.10991802639028869No Hit
CCCAGGAACGGCCCCCAAGCTCCTCATTTATAAGAATAGTCAAGGGCCCTCAGGGGTCCCTGACCGATTCTCTGG6610.10975198707549973No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC6400.10626516146493166No Hit
GTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGA6370.10576704352056479No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6370.10576704352056479No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT6340.10526892557619794No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCCTTG6270.10410665037267523No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6260.10394061105788628No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6140.1019481392804188No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6100.101283982021263No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC6080.10095190339168508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)