FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602267
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75001.2452948609171681No Hit
GACTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG34350.570345046300063No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24240.40247929904842866No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC23790.39500752988292565No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGTCATCCCATG20090.3335729834110121No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19120.3174671698764833No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17750.2947197837503964No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCT16480.27363279077219904No Hit
CTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCG15600.2590213310707709No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14590.24225136027708638No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14090.23394939453763863No Hit
GTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG12590.20904349731929528No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12360.20522459307914928No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12300.20422835719041554No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12200.202567964042526No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12110.2010736102094254No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12100.20090757089463643No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG12080.2005754922650585No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11790.1957603521361788No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11740.19493015556223403No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11490.19077917269251013No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11460.19028105474814325No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11450.1901150154333543No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11420.18961689748898744No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11350.18845462228546475No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC10700.17766206682418262No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10170.168861983140368No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10140.1683638651960011No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9800.1627185284931766No Hit
GCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG9740.16172229260444287No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9340.15508072001288464No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9250.15358636617978405No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC9160.15209201234668346No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG8950.1486051867361154No Hit
GTCAAGGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGAC8760.14545043975512523No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG8720.1447862824959694No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8430.13997114236708968No Hit
CCCAGGAACGGCCCCCAAGCTCCTCATTTATAAGAATAGTCAAGGGCCCTCAGGGGTCCCTGACCGATTCTCTGG8090.1343258056642652No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8060.1338276877198983No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG8050.13366164840510936No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7990.13266541251637565No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCATCTCCCAGGAACGGCCCC7980.13249937320158667No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7870.13067294073890817No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC7850.13034086210933024No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7810.1296767048501744No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7700.1278502723874959No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC7550.12535968266566158No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7490.12436344677692783No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7300.12120869979593768No Hit
GAATAGTCAAGGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGGCCAT7230.120046424592415No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT7230.120046424592415No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT7150.11871811007410334No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7150.11871811007410334No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGACTATTACTGTGCAG7130.11838603144452542No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7090.11772187418536961No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC6710.11141238022338928No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTAT6700.11124634090860033No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCCTTG6630.11008406570507764No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGC6610.10975198707549973No Hit
CCCCTGAGGGCCCTTGACTATTCTTATAAATGAGGAGCTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTATA6530.1084236725571881No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6460.10726139735366541No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6280.1042726896874642No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6270.10410665037267523No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG6220.10327645379873047No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACC6130.10178209996562987No Hit
CTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC6120.10161606065084092No Hit
GTATACATAATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA6100.101283982021263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCTA150.002037363471.5667855
AGGCGTA200.0065843553.2228852
AATGTTG250.00971548348.23340268
ATGTTGA406.491466E-445.73198769
TCACACG307.074789E-444.9427829
GTGGTAT1500.042.532531
CGTGCAA350.00109281741.1429157
GACAGGT1050.038.375221
TCTTCGG1603.3844117E-837.5061770
GTATCAA9350.036.9900171
GGTATCA4000.036.096691
TGGTATC1800.035.4320072
CGGTACC400.002483939834.80985645
GGAGTAC400.002963807733.5783163
TTTATTC1203.6379788E-1233.5783164
ATCTTCG1109.763971E-833.2596369
GCCAGAG9350.032.89311270
AAGGTAG450.003339227332.7703563
AAAGGTA450.003409926832.63051262
TATTCAG1255.456968E-1232.2351846
Produced by FastQC (version 0.11.5)