FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004689532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522024
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58800.3863276794584054No Hit
GTATGGTACCTCACCCCTCACCTTCGGCCAAGGGACACGGCTGGAGATTAGACGAACTGTGGCTGCACCAT47790.31398979253940806No Hit
CTCCTGTACAGCCTCTGGATTCATGTTTAGCAGCCGTGCCATGAGTTGGG46640.3064340641146263No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41700.27397728288121603No Hit
GTACCATACTGCTGACAGGAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTC37160.2441485810999038No Hit
GTATATTCCTGTCAGCAGTATGGTACCTCACCCCTCACCTTCGGCCAAGGGACACGGCTGGAGATTAGACGAA30360.1994712304142379No Hit
GTAATATAAGGCCGAGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAATA30120.19789438274297907No Hit
GGAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28900.1898787404140802No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28280.1858052172633283No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTGTTT28110.18468828349618666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27680.1818630980851813No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25170.1653718995232664No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25030.1644520717150321No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23620.15518809164638664No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23520.15453107178336215No Hit
CAGTAATATAAGGCCGAGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAA23470.1542025618518499No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23060.15150878041344948No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22680.14901210493395636No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCCCCTGCAGGGCCAGTCAGA21050.13830268116665703No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20600.13534609178304677No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20130.13225809842683164No Hit
ATATTACTGTGCGAAAGACTTAATTTCGTACGTGGGGGCGTCCTTCTTTGACTGCTGGGGCCGGGGAACCCTGGT20110.13212669445422673No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19340.1270676415089381No Hit
GTCTGGGACAGACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATT18670.12266560842667397No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18660.1225999064403715No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18400.1208916547965078No Hit
TTATATTACTGTGCGAAAGACTTAATTTCGTACGTGGGGGCGTCCTTCTT18340.1204974428786931No Hit
CCCCTGCAGGGCCAGTCAGAGTGTGAATAGTAACTACTTAGCCTGGTACC18290.12016893294718085No Hit
GTGTGAATAGTAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT18180.11944621109785392No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17970.11806646938550246No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCCCCT17940.11786936342659511No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17780.11681813164575591No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17640.11589830383752162No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGAGATGCCAGTGGCCCTCCT17640.11589830383752162No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17300.11366443630323833No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCCCCTGC17290.11359873431693587No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17250.11333592637172607No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17030.11189048267307217No Hit
GCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGT16830.11057644294702317No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGAGATGCCA16770.11018223102920847No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16660.10945950917988154No Hit
GTATTAGTGGTGGTGCTGGTAGCACCTCCTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT16340.10735704561820313No Hit
GACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTATATTCCTGTCAGCAGTATGGTACCTCA16250.10676572774148108No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16230.10663432376887619No Hit
GCCTTATATTACTGTGCGAAAGACTTAATTTCGTACGTGGGGGCGTCCTTCTTTGACTGCTGGGGCCGGGGAACC16220.10656862178257374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16130.10597730390585168No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15790.1037434363715684No Hit
GGGTTGGAGTGGGTCTCAGGTATTAGTGGTGGTGCTGGTAGCACCTCCTA15480.10170667479619244No Hit
CTCCAACCCCTTCCCTGGAGCCTGGCGGACCCAACTCATGGCACGGCTGC15390.10111535691947038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)