FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597659
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC46560.7790395526546073No Hit
GTTTTATACAGCTCCAATGAGAGGAACTACTTAGCTTGGTACCAGCAGAA44720.7482527662094941No Hit
ATATTATAGTGCCCCTCAAACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT38680.6471917933135785No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27260.4561129339640163No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22880.38282699666532255No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA21500.3597369068314875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18590.31104693479057455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18040.3018443627553504No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17670.2956535415680179No Hit
AAGTAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAA16810.2812640652947584No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCGGCCA15180.25399098817218513No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15120.25298707122288794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14610.244453777153862No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13570.22705255003271094No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA12860.2151728661326944No Hit
GTTTATTACTGTCAGCAATATTATAGTGCCCCTCAAACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAA12160.20346050172422736No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12010.2009507093509844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11550.1932540127397061No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA11460.19174813731576032No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11420.1910788593495622No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11130.18622659409462589No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10970.18354948222983342No Hit
GTCCCAGACCCGCGGCCACTGAATCGCTCAGGGACCCCGGATTCCCGGGT10950.18321484324673434No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10950.18321484324673434No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTT10840.1813743288396895No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10800.18070505087349142No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10560.1766893830763027No Hit
ACTTAGGAGGCTGTCCTACTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCTCATTGGAGCTGTATAAAACACT10320.17267371527911402No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10230.17116783985516826No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCGGCCACTGAATCGCTCAGGGACC10090.16882536697347483No Hit
GAGTGAAATCTGTCCCAGACCCGCGGCCACTGAATCGCTCAGGGACCCCG9980.16698485256643003No Hit
GAACTACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGTTAC9880.16531165765093472No Hit
GTCCTACTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCTCATTGGAGCTG9690.1621325873114937No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCGGCCA9590.16045939239599838No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9460.1582842390058545No Hit
GCTCCAATGAGAGGAACTACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGTTACTCATTTACTGGG9220.1542685712086658No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9220.1542685712086658No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC9180.15359929324246768No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGCC9160.15326465425936864No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9150.15309733476781912No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9130.15276269578472004No Hit
CTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT9040.15125682036077429No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAATGAGAGGAACTA8930.14941630595372948No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8930.14941630595372948No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8770.14673919408893701No Hit
ATTCAGTGGCCGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC8550.14305816527484735No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8440.14121765086780255No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC8050.1346921906973709No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8000.13385559323962326No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7940.13285167629032607No Hit
GTCCCTGAGCGATTCAGTGGCCGCGGGTCTGGGACAGATTTCACTCTCAC7850.1313458008663803No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT7680.12850136951003832No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7600.12716281357764211No Hit
ACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGTTACTCATT7540.12615889662834492No Hit
CTACTTAGCTTGGTACCAGCAGAAAGTAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATC7340.12281250679735435No Hit
GTAAATGAGTAACTTAGGAGGCTGTCCTACTTTCTGCTGGTACCAAGCTA7330.12264518730580481No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7160.11980075594946282No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7040.11779292205086847No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7020.11745828306776941No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6940.11611972713537319No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6850.11461385171142742No Hit
CTTAGGAGGCTGTCCTACTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCT6740.1127733373043826No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG6720.11243869832128353No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6710.11227137882973402No Hit
AACCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT6640.11110014238888731No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6580.11009622543959013No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTTTGAGGGGCA6560.10976158645649106No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6480.10842303052409484No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6430.1075864330663472No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6430.1075864330663472No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6430.1075864330663472No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6390.10691715510014908No Hit
CTCCAGGTTGGTCCCCTGGCCAAAAGTTTGAGGGGCACTATAATATTGCT6390.10691715510014908No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG6340.10608055764240144No Hit
ATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGC6240.10440736272690615No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6190.10357076526915851No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6170.10323612628605945No Hit
ACCCGGGAATCCGGGGTCCCTGAGCGATTCAGTGGCCGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC6120.10239952882831181No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG6100.10206488984521274No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6090.1018975703536632No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6090.1018975703536632No Hit
TTATTACTGTCAGCAATATTATAGTGCCCCTCAAACTTTTGGCCAGGGGA6080.10173025086211368No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6080.10173025086211368No Hit
GCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGAGCGATTCAGTGGCCGCGGGT6050.1012282923874651No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGGTCCGGCAGCC5990.10022437543816792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTT100.0069977576145.6236670
CGTCTTT300.001901612572.8118370
CTCGGGT150.001986853372.02109560
TTGTGCC200.0058675654.79625764
ATCGCGT352.4006275E-548.8271337
GTAACCA450.00946993348.54121870
GGTATCA4850.046.7541661
CATCGCG504.1278327E-640.9663336
CGTATGC1350.040.76328741
CTTATAC3250.039.5719531
GCGTGAA458.395358E-539.4949256
TATACAC3450.038.2589233
TCGTATG1350.038.1507140
ACGAGAC3550.038.15052822
CACGAGA3550.038.1473421
ATCTCGT1650.037.28616737
TACACAT3850.036.92125
CGAGACT3500.036.7607623
ATATCTT400.001915371236.70318665
CGAGCCC3550.036.23087715
Produced by FastQC (version 0.11.5)