FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155597
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT58340.5048472780735845No Hit
GTTTTATACAGCTCCAACAGTAAGAATTACTTAGCTTGGTACCAGCAGAA51490.44557055790210603No Hit
GCCTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCTTACTGTTGGAGCTGTATAAAACATT50690.43864772926894063No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43740.3785056555183165No Hit
ATATTATAGTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT42820.37054440259017635No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37780.3269305822012345No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29600.2561446594271186No Hit
GTATAAAACATTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA28850.2496545075835261No Hit
CTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC28660.24801033578314932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28390.24567388111945598No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27470.23771262819131583No Hit
TTATAGTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24580.2127039097540059No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24180.20924249543742326No Hit
GTTTATTACTGTCACCAATATTATAGTACTCCTCCGTGGACGTTCGGCCA24010.20777139435287562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23060.19955053535099174No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23000.1990313232035043No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22610.19565644424483622No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22060.190896999559535No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21100.18258960519973658No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20800.17999354446229957No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20470.17713787765111885No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20380.17635905942988775No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20230.17506102906116927No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19820.171513079386672No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAATGTTTTATA19770.17108040259709917No Hit
GTACTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT19770.17108040259709917No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAA19750.17090733188127002No Hit
GAGGAGTACTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCC19720.17064772580752632No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19050.1648498568272503No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18650.16138844251066764No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAA18640.16130190715275305No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18120.1568020685411956No Hit
CTCTTGTACAGACTCTGGATTCACATTCAGTAACGCCTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG17210.14892735097096998No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16870.14598514880187471No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16570.14338908806443768No Hit
GAATTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAGGCTGC16350.1414853101903172No Hit
GTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA16310.14113916875865892No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16150.13975460303202586No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16020.1386296433791365No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATAGTACT16000.13845657266330738No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15710.13594704728378493No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15680.13568744121004123No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15580.13482208763089557No Hit
GTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA15540.13447594619923728No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15480.13395673405174988No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15290.13231256225137308No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT15180.13136067331431286No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15120.13084146116682546No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15050.1302357136614235No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14950.1293703600822778No Hit
GGATCTCTGGTGCCTACGGCGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14470.1252166629023786No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGCTGGTA14240.12322634967034354No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13990.12106296572247939No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGC13910.12037068285916284No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC13910.12037068285916284No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAACAGTAAGAATTA13900.12028414750124827No Hit
GCTCCAACAGTAAGAATTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGG13820.11959186463793173No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13740.11889958177461521No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC13520.11699580390049473No Hit
GCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT13110.11344785422599747No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13060.11301517743642464No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13000.11249596528893724No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12770.11050565205690217No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12650.10946722776192738No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12630.10929415704609825No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12620.1092076216881837No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12500.10816919739320888No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCTTACTGTTGGAGCTG12480.10799612667737975No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12460.10782305596155062No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAGGAGTACTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAG12400.1073038438140632No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12250.10600581344534471No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12220.105746207371601No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12090.10462124771871163No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11990.10375589413956596No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11780.10193865162336005No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA11770.10185211626544549No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11670.10098676268629982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)