FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155597
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT50110.4336286785098957No Hit
GCCTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCTTACTGTTGGAGCTGTATAAAACATT45870.3969376867541193No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45570.39434162601668227No Hit
ATATTATAGTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT43300.3746980997700755No Hit
GTTTTATACAGCTCCAACAGTAAGAATTACTTAGCTTGGTACCAGCAGAA41840.3620639375145488No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33140.28677817612887535No Hit
CTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC32610.2821918021594033No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29720.2571830837220934No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29590.2560581240692041No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28730.2486160832885513No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA24810.214694222986041No Hit
TTATAGTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT23990.2075983236370465No Hit
GTTTATTACTGTCACCAATATTATAGTACTCCTCCGTGGACGTTCGGCCA23940.20716564684747363No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23150.20032935357222284No Hit
GTACTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT23000.1990313232035043No Hit
GTATAAAACATTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA22980.1988582524876752No Hit
GAGGAGTACTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCC21720.18795479739043974No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21710.18786826203252519No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21690.18769519131669604No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21320.18449338307385707No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21320.18449338307385707No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20590.17817630194609366No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20340.1760129179982295No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19920.17237843296581767No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19860.17185922081833027No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18960.16407103860601924No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18680.16164804858441134No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18290.15827316962574323No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18280.15818663426782867No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17770.15377333101418575No Hit
CTCTTGTACAGACTCTGGATTCACATTCAGTAACGCCTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG17640.1526483713612964No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17230.14910042168679913No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17040.14745624988642234No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAATGTTTTATA16930.1465043609493621No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16710.14460058307524162No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAA16490.14269680520112116No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT16270.1407930273270007No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATAGTACT15950.13802389587373454No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15910.13767775444207625No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15810.1368124008629306No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG15510.13421634012549358No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAA15240.13187988546180027No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC15140.13101453188265458No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15110.1307549258089109No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14950.1293703600822778No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14810.12815886507147387No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14750.1276396529239865No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14660.12686083470275536No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14650.1267742993448408No Hit
GTACTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA14130.12227446073328331No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14090.12192831930162504No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14030.12140910715413764No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14000.12114950108039395No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13920.1204572182170774No Hit
GTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA13630.11794769283755496No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13620.1178611574796404No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13590.11760155140589669No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13500.11682273318466559No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAGGAGTACTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAG13440.11630352103717818No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13400.11595737960551991No Hit
GGATCTCTGGTGCCTACGGCGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13190.114140137089314No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13170.11396706637348487No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGCTGGTA12980.1123228945731081No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12890.111544076351877No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12760.11041911669898762No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC12740.11024604598315849No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12490.1080826620352943No Hit
GAATTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAGGCTGC12410.10739037917197779No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAATTCTTACTGTTGGAGCTG12100.1047077830766262No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATAGTACTCCTC11940.10332321734999313No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11940.10332321734999313No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11930.10323668199207856No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11920.10315014663416398No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11890.10289054056042028No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGC11780.10193865162336005No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAACAGTAAGAATTA11590.10029447982298327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGTCG252.3080087E-456.40186756
GTGGTAT2050.044.8444371
GGTATCA8600.044.3423041
TTTGTCG350.001437660738.9143188
TGGTATC2500.038.1327322
GACAGGT1700.038.0542681
GTATCAA20450.036.629251
GCTCGGG3500.034.65720470
CATTTAG400.00276954934.0485531
GCTGCAT850.002963764633.57791570
TATCAAC24450.030.2176552
ATCAACG24200.029.9644643
ATGCTCC803.0591746E-629.7937748
TCAACGC24400.029.5844524
GGTCTGG28550.029.24101470
CAACGCA24750.029.1660845
AACGCAG24750.029.1660846
GTAGTTT1255.6546426E-428.54122770
AGGTATA605.861028E-428.3701113
ATTGGGC755.322193E-528.20093356
Produced by FastQC (version 0.11.5)