FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126576
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29310.26016886566019515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28180.25013847268182526No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCAAGATATA23680.21019442984760903No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT20760.18427518427518427No Hit
GTAATAAACCCCGACATCTTCAGCTTCCACCCTGCTGATTTTTAGTGTGAAATCTGTCCCTGCCCCACTGCCA20420.18125718992771017No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19610.17406726221755123No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18850.16732115720555027No Hit
GTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATCAGGTTT18130.16093011035207566No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17900.15888852594054906No Hit
GTTTATTACTGCATGCAATCTACACAATTTCCTCATCTCACTTTCGGCGG17800.15800088054423314No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17620.1564031188308645No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17070.15152106915112695No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGGCTCGTA17000.1508997173737058No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16900.1500120719773899No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16730.14850307480365282No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15840.14060303077644118No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT15600.138472681825283No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15470.13731874281007228No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15160.13456704208149295No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14750.1309276959565977No Hit
GTGTATTACTGTGCGAGGTATTTCGATTGTCTTAGTACGAGCTGCTCCACGGACGATTCTTTTGATATCTGGGGC14720.13066140233770293No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14390.12773217252986038No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14360.1274658789109656No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATCAGGTTTCCAACCGGTTC13960.12391529732570196No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13720.12178494837454376No Hit
GTCTGGGACCCCAGAGAACCGGTTGGAAACCTGATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAAG13450.11938830580449078No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA13450.11938830580449078No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13320.1182343667892801No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13280.11787930863075372No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13270.11779054409112213No Hit
CTCCTGTGCAGCCTCTGGGATCATCGTCAGTAGCAACTACATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG13240.11752425047222735No Hit
GTATTACTGTGCGAGGTATTTCGATTGTCTTAGTACGAGCTGCTCCACGG13180.1169916632344378No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12790.11352984618880571No Hit
CTCCTCACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA12020.10669497663717317No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11810.10483092130490973No Hit
CCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCT11740.10420956952748861No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCTAGAGACAATTCCAAGAACACACTATATCTTGAAATGAA11710.10394327590859383No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11690.10376574682933065No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTAAA11420.10136910425927767No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11370.10092528156111971No Hit
CAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGGGGATATTGTGCTGA11330.10057022340259335No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11320.10048145886296177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)