FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126576
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70150.6226832455156155No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28810.25573063867861556No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCAAGATATA21810.1935954609365014No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21590.19164264106460638No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21250.18862464671713228No Hit
GTAATAAACCCCGACATCTTCAGCTTCCACCCTGCTGATTTTTAGTGTGAAATCTGTCCCTGCCCCACTGCCA20990.18631676868671088No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18610.16519080825439206No Hit
GTTTATTACTGCATGCAATCTACACAATTTCCTCATCTCACTTTCGGCGG17180.15249747908707448No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGGCTCGTA16230.14406484782207327No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16110.14299967334649416No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15630.13873897544417776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15490.13749627188933547No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15450.13714121373080912No Hit
GTGTATTACTGTGCGAGGTATTTCGATTGTCTTAGTACGAGCTGCTCCACGGACGATTCTTTTGATATCTGGGGC15220.1350996293192825No Hit
GTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATCAGGTTT14730.1307501668773345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14700.13048387325843971No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT14430.12808723068838676No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA14420.12799846614875515No Hit
CTCCTGTGCAGCCTCTGGGATCATCGTCAGTAGCAACTACATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG14080.12498047180128104No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14060.12480294272201786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13450.11938830580449078No Hit
GTATTACTGTGCGAGGTATTTCGATTGTCTTAGTACGAGCTGCTCCACGG13380.11876695402706963No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13170.1169028986948062No Hit
GTCTGGGACCCCAGAGAACCGGTTGGAAACCTGATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAAG13160.11681413415517462No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12920.11468378520401643No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12230.1085590319694366No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATCAGGTTTCCAACCGGTTC12020.10669497663717317No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11960.10616238939938362No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11800.10474215676527815No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11720.10403204044822544No Hit
CTCCTCACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA11440.10154663333854085No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11370.10092528156111971No Hit
CCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCT11360.10083651702148813No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA300.002153383370.57397570
GCGTCTA150.00236763968.9107939
GGTATCA12800.044.4856341
GTATCAA32700.038.5580981
TGGTATC3400.037.8796352
GTGGTAT3350.037.434961
CTAATCG400.002251471935.51689557
CGAAACC751.1304008E-535.37572569
ATCAACG37150.033.8527073
TATCAAC37200.033.80122
CCGAAAC701.6148262E-533.58112368
TCAACGC37450.033.315894
AACGCAG38300.032.83776
CAACGCA38100.032.8321275
ACCGCGC450.003580098532.31036865
TGCGGTA552.7466376E-432.28808257
CGCAGAG39650.031.976148
TAATCGA450.00396458231.64420158
ACGCAGA39750.031.6398477
AGAGTAC40900.030.9878611
Produced by FastQC (version 0.11.5)