FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938903
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATATACAGTGATGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCC63150.6725934414950213No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40900.43561475466581745No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35560.3787398698268085No Hit
GTATATGAGAGCCTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA34150.36372234405471066No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGGCTCTCATA30410.3238886232124085No Hit
CATATACAGTGATGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCCAG29610.3153680412140551No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27380.29161691889364505No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27290.2906583534188303No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT25860.27542781309677355No Hit
GTAATAAACCCCGACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGTCTGACCCACTGCCG25800.27478876944689706No Hit
CTTTTGGCCAGGGGACCAACCTCGAGATCAAACGAACTGTGGCTGCACCA24010.25572396722558133No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23050.2454992688275573No Hit
GTTTATTACTGCATGCAAGGAACACACTGGCCGTACACTTTTGGCCAGGGGACCAACCTCGAGATCAAACGAA22110.23548758497949204No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22100.2353810777045126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21420.2281385830059122No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21270.22654097388122096No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20280.2159967536582586No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20210.2152512027334027No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19590.20864775168467883No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18990.20225731518591378No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18870.20097922788616077No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC18650.19863606783661358No Hit
GGCCAAAAGTGTACGGCCAGTGTGTTCCTTGCATGCAGTAATAAACCCCG18560.19767750236179882No Hit
GTGTGTTCCTTGCATGCAGTAATAAACCCCGACATCCTCAGCCTCCACCC18260.1944822841124163No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18230.19416276228747803No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA17840.19000897856328078No Hit
GTCTGGGACCCCAGAGTCCCGGTCGGAAAGCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA17570.18713328213883648No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGAGGTTGGTCC17380.18510964391422757No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGCTTTCCGACCGGGAC17030.18138188928994795No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16940.18042332381513318No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15510.1651927834930765No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT15300.16295613071850873No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC15120.1610389997688792No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC14980.15954789791916738No Hit
CTGTATATGAGAGCCTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC14620.15571363601990834No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14510.15454205599513474No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14390.15326396869538172No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGACACTGATTTCACACTGAA14190.15113382319579338No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13960.1486841558712668No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13610.14495640124698717No Hit
GGTCTAGTCAGGCTCTCATATACAGTGATGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCCAGGCCAATCTC13530.14410434304715183No Hit
GTGCAGCCACAGTTCGTTTGATCTCGAGGTTGGTCCCCTGGCCAAAAGTG13350.1421872120975223No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12940.1378204138233662No Hit
GGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGACACTGATT12240.13036490457480698No Hit
GAACACACTGGCCGTACACTTTTGGCCAGGGGACCAACCTCGAGATCAAA12220.13015189002484814No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGGCTCTCATATA12110.12898031000007454No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12110.12898031000007454No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11900.1267436572255068No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11830.12599810630065086No Hit
GGAACACACTGGCCGTACACTTTTGGCCAGGGGACCAACCTCGAGATCAAACGAACTGTGGCTGCACCAT11720.12482652627587727No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11720.12482652627587727No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11640.12397446807604193No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11570.12322891715118602No Hit
GGGTCAGACACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGA11570.12322891715118602No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11500.12248336622633009No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11340.12077924982665941No Hit
CTCATATACAGTGATGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATT10950.11662546610246213No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10770.11470833515283262No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10730.11428230605291495No Hit
GTGTACGGCCAGTGTGTTCCTTGCATGCAGTAATAAACCCCGACATCCTC10690.11385627695299728No Hit
TTTATAAGCTTTCCGACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGACACTGATTTCACA10670.11364326240303843No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10600.1128977114781825No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10490.11172613145340893No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10440.11119359507851184No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10400.11076756597859416No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10300.10970249322879999No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10250.1091699568539029No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10200.10863742047900582No Hit
GTGATGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGCTTT10110.10767885500419107No Hit
TTATTACTGCATGCAAGGAACACACTGGCCGTACACTTTTGGCCAGGGGA10100.10757234772921165No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10020.10672028952937632No Hit
ATTTATAAGCTTTCCGACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGACACTGATTTCACA9950.10597473860452038No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9940.10586823132954096No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT9910.10554870950460271No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9810.10448363675480854No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9790.1042706222048497No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9770.10405760765489086No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9690.10320554945505553No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9680.10309904218007611No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA9560.10182095488032311No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9460.10075588213052891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)