FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938903
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATATACAGTGATGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCC57060.6077305110325562No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53250.567151239265398No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40520.43156747821659963No Hit
GTATATGAGAGCCTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA31490.3353914089101856No Hit
GTAATAAACCCCGACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGTCTGACCCACTGCCG28870.3074865028655782No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26670.2840549023701064No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25640.2730846530472264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25060.2669072310984202No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGGCTCTCATA24750.2636055055740582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23140.24645783430237203No Hit
CATATACAGTGATGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCCAG23030.24528625427759845No Hit
GTTTATTACTGCATGCAAGGAACACACTGGCCGTACACTTTTGGCCAGGGGACCAACCTCGAGATCAAACGAA22500.23964136870368932No Hit
CTTTTGGCCAGGGGACCAACCTCGAGATCAAACGAACTGTGGCTGCACCA22050.23484854132961552No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21040.22409130655669435No Hit
GTGTGTTCCTTGCATGCAGTAATAAACCCCGACATCCTCAGCCTCCACCC21020.2238782920067355No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20530.21865943553274406No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19670.20949980988451417No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19460.2072631571099464No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC19290.2054525334352963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19200.20449396796048155No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17680.1883048621636101No Hit
GTCTGGGACCCCAGAGTCCCGGTCGGAAAGCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA17660.18809184761365125No Hit
GGCCAAAAGTGTACGGCCAGTGTGTTCCTTGCATGCAGTAATAAACCCCG17590.18734629668879535No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16880.1797842801652567No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16490.1756304964410594No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16250.17307432184155339No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15880.16913355266731495No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGAGGTTGGTCC15790.16817498719250018No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14620.15571363601990834No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14600.1555006214699495No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA14570.15518109964501126No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14530.1547550705450936No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14040.14953621407110212No Hit
GTGCAGCCACAGTTCGTTTGATCTCGAGGTTGGTCCCCTGGCCAAAAGTG13780.14676702492163726No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGCTTTCCGACCGGGAC13600.14484989397200776No Hit
GAACACACTGGCCGTACACTTTTGGCCAGGGGACCAACCTCGAGATCAAA13310.14176118299760465No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13250.14112213934772813No Hit
CTGTATATGAGAGCCTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC13200.14058960297283105No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT13090.13941802294805747No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC13020.13867247202320154No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC12930.13771390654838678No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12800.13632931197365436No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGACACTGATTTCACACTGAA12500.13313409372427185No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12250.13047141184978642No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12120.12908681727505397No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12100.12887380272509513No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11890.12663714995052738No Hit
GGTCTAGTCAGGCTCTCATATACAGTGATGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCCAGGCCAATCTC11810.12578509175069202No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11750.12514604810081553No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11740.1250395408258361No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGGCTCTCATATA11540.12290939532624776No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11530.12280288805126834No Hit
GGAACACACTGGCCGTACACTTTTGGCCAGGGGACCAACCTCGAGATCAAACGAACTGTGGCTGCACCAT11370.12109877165159767No Hit
GGGTGGAGGCTGAGGATGTCGGGGTTTATTACTGCATGCAAGGAACACACTGGCCGTACACTTTTGGCCAGGGGA11370.12109877165159767No Hit
GTGTACGGCCAGTGTGTTCCTTGCATGCAGTAATAAACCCCGACATCCTC11330.12067274255167998No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA11220.11950116252690639No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10940.11651895882748271No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10890.11598642245258563No Hit
GGGTCAGACACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGA10880.11587991517760621No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10620.11311072602814135No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10440.11119359507851184No Hit
GGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGACACTGATT10310.10980900050377941No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10170.10831789865406757No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10170.10831789865406757No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10090.10746584045423221No Hit
TTATTACTGCATGCAAGGAACACACTGGCCGTACACTTTTGGCCAGGGGA9930.10576172405456154No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9810.10448363675480854No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9810.10448363675480854No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9700.10331205673003495No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9490.10107540395546719No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT9480.10096889668048777No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9420.10032985303061125No Hit
CAGTAATAAACCCCGACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGTCTGACCCACTGCCG9410.10022334575563183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT300.002024185171.68140470
GGTATCA10650.041.0956761
GTGGTAT2550.038.5845871
CGGGTAT451.0960661E-437.74986617
GTATCAA27150.037.489311
TGGTATC2900.035.103362
GACAGGT2900.035.097751
CGAAATT501.7436812E-434.8847748
CTAATTT24700.032.213170
TATCAAC31900.032.1248932
ATCAACG32200.031.7269733
TCAACGC32800.031.2484114
AACGCAG33200.031.17866
CAACGCA33050.031.1147295
TATTCTA1902.2889981E-730.18164470
ACGCAGA34600.029.8205387
CGCAGAG34850.029.5092288
AGTTTAT2650.029.45152
AGAGTAC35200.029.11318411
GCAGAGT35650.028.8454919
Produced by FastQC (version 0.11.5)