FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128273
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52010.4609699957368474No Hit
GTAATATACAGCCGTGTCTTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA44300.39263547031613805No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41370.36666657803563496No Hit
GTATATTGCTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGTCGCCTAAGTTG36500.323503265610362No Hit
CTCTATGAGAGGTCGAATAACCATCACCCCAGACACATCCATGAACCAGTTCTCCCTGCAGCTGAACTCTGTGA34620.3068406316556365No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32650.2893803184158444No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31610.28016269112174097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30190.26757708462402274No Hit
GTATATTACTGTGCAAGAGGGGTCATTACTGGAACTCCGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC27530.2440012301987196No Hit
ATATTGCTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA27000.23930378551999384No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26090.2312383616376533No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25720.22795901346571262No Hit
AAGCAGGACAGTCGCCTAAGTTGCTCATTTACTGGGCATCAACCCGGGAA25530.2262750238639053No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT25420.22530008251549047No Hit
GAGTATATTGCTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGC25000.22157757918517948No Hit
CTCATAGAGAGTGCATAATCATTAGACCACTTGGACCTGTAGTACGTCCT23590.20908060371913534No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23380.20721935205397982No Hit
ATATTATGATACCCCGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23360.2070420899906317No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22930.2032309556286466No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21260.18842957333907664No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20980.1859479044522026No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20630.1828458183436101No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19960.17690753922144728No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19850.1759325978730325No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19650.17415997723955107No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC19540.17318503589113626No Hit
GTATACGGGGTATCATAATATTGGTGACAGAAATAAACTGCCACATCTTC19330.17132378422598077No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA19140.16963979462417342No Hit
CCCATGGGGTGTCCTGTCACAGGTACAACTGCAGGAGTCAGGTCCAGGAC19050.16884211533910676No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATATTGCTCAGCTCCAA18970.16813306708571418No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18890.1674240188323216No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18810.16671497057892903No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18540.16432193272372908No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCACC18490.16387877756535874No Hit
ATGATACCCCGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA17680.15669966399975893No Hit
GTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG17670.15661103296808485No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATGATACC17660.1565224019364108No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17600.15599061574636636No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17420.15439525717623306No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16920.14996370559252947No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16770.14863424011741838No Hit
TCATAATATTGGTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC16630.1473934056739814No Hit
CGCCTAAGTTGCTCATTTACTGGGCATCAACCCGGGAATCCGGGGTCCCT16540.14659572638891474No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC16470.14597530916719623No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16360.14500036781878145No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16320.14464584369208516No Hit
GGGTATCATAATATTGGTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT16300.14446858162873702No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATATTGCT16220.14375953337534444No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16170.14331637821697407No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCC15850.1404801852034038No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15540.13773262322150756No Hit
ACTTAGGCGACTGTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAGCAATATACT15410.1365804198097446No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATATTGCTCAGCTCCAACAATAAGAACTA15140.1341873819545447No Hit
GTCAGGGACCCCGGATTCCCGGGTTGATGCCCAGTAAATGAGCAACTTAGGCGACTGTCCTGCTTTCTGCTGGTA15130.1340987509228706No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15120.13401011989119654No Hit
GGCCAAAAGTATACGGGGTATCATAATATTGGTGACAGAAATAAACTGCC14960.1325920233844114No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14870.13179434409934476No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14860.13170571306767068No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14830.13143981997264848No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14640.1297558303708411No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14500.1285149959274041No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14070.12470386156541902No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13640.12089272720343391No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13560.12018367895004134No Hit
GTTTATTTCTGTCACCAATATTATGATACCCCGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13520.11982915482334505No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13320.11805653418986363No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13150.11654980665140441No Hit
CTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGTCGCCTAAGTTGCTCATTTACTGGGCATCAACCCGGGAATC12990.11513171014461926No Hit
GCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGTCGCCTAAGTTGCTCATTTACTGGG12970.1149544480812711No Hit
ACTTAGCTTGGTACCAGCAGAAAGCAGGACAGTCGCCTAAGTTGCTCATT12680.11238414816272303No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG12310.10910479999078238No Hit
CCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCACCAA12260.10866164483241202No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG12220.10830712070571573No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12130.1075094414206491No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12020.10653450007223428No Hit
GTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG12000.10635723800888615No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGC12000.10635723800888615No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11980.106179975945538No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11950.10591408285051578No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11940.1058254518188417No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11830.10485051047042693No Hit
GAGCAATATACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA11810.10467324840707878No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11790.10449598634373065No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11790.10449598634373065No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11760.10423009324870842No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11430.10130526920346405No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11410.10112800714011591No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11390.10095074507676777No Hit
GTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATG11360.10068485198174554No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATGATACCCCGTATACTTTTGGCCAGGGGACCAAG11340.10050758991839741No Hit
GCAATATACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA11330.10041895888672334No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11290.10006443476002706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)