FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128273
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61640.5463216792389785No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50520.44776397201741064No Hit
GTAATATACAGCCGTGTCTTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA40370.3578034748682278No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35420.3139311141895623No Hit
CTCTATGAGAGGTCGAATAACCATCACCCCAGACACATCCATGAACCAGTTCTCCCTGCAGCTGAACTCTGTGA34540.30613158340224395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31440.2786559635832817No Hit
GTATATTGCTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGTCGCCTAAGTTG31170.27626292572808175No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30020.26607035708556354No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29120.25809356423489704No Hit
GTATATTACTGTGCAAGAGGGGTCATTACTGGAACTCCGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC27390.24276039575528266No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27050.23974694067836422No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24420.21643697934808334No Hit
ATATTATGATACCCCGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23450.20783976927569833No Hit
ATATTGCTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA23030.20411726594538734No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22270.19738130753815786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22190.1966722592847653No Hit
GAGTATATTGCTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGC21720.19250660079608392No Hit
CTCATAGAGAGTGCATAATCATTAGACCACTTGGACCTGTAGTACGTCCT21020.1863024285788989No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA20150.17859152882325466No Hit
GTATACGGGGTATCATAATATTGGTGACAGAAATAAACTGCCACATCTTC19930.17664164612642508No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19890.1762871219997288No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19810.17557807374633622No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19680.1744258703345733No Hit
AAGCAGGACAGTCGCCTAAGTTGCTCATTTACTGGGCATCAACCCGGGAA19640.17407134620787698No Hit
GGGTATCATAATATTGGTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT19320.1712351531943067No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19300.17105789113095854No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19250.1706147359725882No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19200.17017158081421782No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19190.17008294978254376No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19050.16884211533910676No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18970.16813306708571418No Hit
CCCATGGGGTGTCCTGTCACAGGTACAACTGCAGGAGTCAGGTCCAGGAC17970.159269963918307No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17970.159269963918307No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17880.15847228463324037No Hit
GTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG17730.15714281915812928No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT17650.1564337709047367No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATGATACC17590.15590198471469227No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17520.15528156749297375No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17120.1517363262260109No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT17070.15129317106764054No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16470.14597530916719623No Hit
ATGATACCCCGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA16450.1457980471038481No Hit
TCATAATATTGGTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC16430.14562078504049994No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG16180.14340500924864816No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16130.14296185409027778No Hit
GGCCAAAAGTATACGGGGTATCATAATATTGGTGACAGAAATAAACTGCC16120.14287322305860373No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCACC16020.141986912741863No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16000.14180965067851486No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATATTGCTCAGCTCCAA15900.14092334036177415No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15720.13932798179164085No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15070.13356696473282617No Hit
GTCAGGGACCCCGGATTCCCGGGTTGATGCCCAGTAAATGAGCAACTTAGGCGACTGTCCTGCTTTCTGCTGGTA15050.13338970266947803No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14910.13214886822604102No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14780.1309966648142781No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCC14550.12895815108577444No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14470.12824910283238186No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATATTGCT14250.1262992201355523No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13940.12355165815365607No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13930.12346302712198201No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13820.12248808577356722No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC13810.12239945474189314No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13630.12080409617175984No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13560.12018367895004134No Hit
ACTTAGGCGACTGTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAGCAATATACT13530.11991778585501912No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATATTGCTCAGCTCCAACAATAAGAACTA13460.11929736863330062No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13380.11858832037990805No Hit
GTTTATTTCTGTCACCAATATTATGATACCCCGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13180.11681569974642662No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13130.11637254458805625No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12750.11300456538444154No Hit
CGCCTAAGTTGCTCATTTACTGGGCATCAACCCGGGAATCCGGGGTCCCT12690.11247277919439709No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12610.11176373094100452No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG12510.11087742062426381No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12390.10981384824417495No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12390.10981384824417495No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATGATACCCCGTATACTTTTGGCCAGGGGACCAAG12240.10848438276906387No Hit
AAAGTATACGGGGTATCATAATATTGGTGACAGAAATAAACTGCCACATC12210.10821848967404167No Hit
GTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG12150.10768670348399723No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12040.10671176213558242No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11890.10538229666047136No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11850.10502777253377507No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11710.10378693809033808No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11700.10369830705866398No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11460.10157116229848628No Hit
GTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATG11440.10139390023513811No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11380.10086211404509371No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11310.10024169682337519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)