FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428779
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67590.47306126419831196No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50270.35183887781105405No Hit
GTATTACTGCCAGCAATATACTACCTCAGGGAGAAATTTTGGCCAGGGGA46440.32503277273812115No Hit
GTGTATTACTGCCAGCAATATACTACCTCAGGGAGAAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA38450.26911089818649353No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33630.23537579989627508No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33460.23418597277815534No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30910.21633856600635926No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29460.20619004058710272No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27910.19534161686306978No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT25040.17525453551598952No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23970.16776562365488293No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22960.16069665077664216No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22740.15915687450613425No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA22630.1583869863708803No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22350.15642727111750662No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22130.15488749484699874No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22090.1546075355250882No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT21440.15005819654404215No Hit
GTCCCAGACCCACTGCCAGTAAACCTGTCTGCGATGCCAGTGGCCCTGCG21160.14809848129066847No Hit
CAACTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCCGCAGGGC21130.14788851179923557No Hit
GTCTCCAGGCTCCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCTGCAGGGCCAGTCAGATTGTTACTCA20780.14543886773251846No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20330.14228932536102504No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCTGCAGGGCCAGTCAGA19440.13606023044851584No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTAAACCTGTCTGCGATGCCA19240.1346604338389632No Hit
GCAATATACTACCTCAGGGAGAAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19170.13417050502561978No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19020.13312065756845531No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18930.13249074909415662No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18490.12941119655314082No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCCGCA18220.12752147113024476No Hit
ATATACTACCTCAGGGAGAAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18110.12675158299499084No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTCTGCAGTGTA17820.12472187791113952No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17810.12465188808066188No Hit
GTCTGGGGCTGAGGTGAAGAAGCCCGGGTCCTCGGTGAAGATCTCCTGCAAGGCTTCTGAAGTCAGCTTCAACAG17800.12458189825018426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17720.1240219796063632No Hit
TCTCTGGTGCATCCCGCAGGGCCACTGGCATCGCAGACAGGTTTACTGGCAGTGGGTCTGGGACAGACTTCACT17420.12192228469203426No Hit
GGTTTGTGCTGGTACCAGGCTAAGTAGTTGTGAGTAACAATCTGACTGGCCCTGCAGGAGACGGTGGCTCTTTCC17390.12171231520060136No Hit
CTCCAGGCTCCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCTGC16770.11737294571098819No Hit
GGTTTACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGA16750.11723296605003293No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15830.11079390164609082No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15710.10995402368035924No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC15540.10876419656223951No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15500.10848423724032898No Hit
GGTTTACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTCTGCAGTGT15430.10799430842698556No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15250.10673449147838819No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15050.10533469486883557No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTT14630.10239512198877503No Hit
CCTCATCTCTGGTGCATCCCGCAGGGCCACTGGCATCGCAGACAGGTTTACTGGCAGTGGGTCTGGGACAGACTT14520.10162523385352107No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14470.10127528470113294No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14400.10078535588778952No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14380.10064537622683424No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14350.10043540673540134No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14310.10015544741349082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.041.5007251
GTATCAA35450.038.8173941
TACGTAT800.002227899435.5913170
TATCAAC41300.032.985352
ATCAACG42500.032.126063
AACGCAG42850.032.1036876
TCAACGC42600.031.9720384
AACTGTG25450.031.88533470
CAACGCA43150.031.6432295
ACGCAGA44800.030.706327
CGCAGAG45300.030.592348
CGGGATA450.00496109530.22736417
AGAGTAC46750.029.70990211
TGTTAGA15150.029.60068970
GCAGAGT47350.029.2678579
GAATACG1254.9039954E-927.1731999
GATTCAC2650.026.925331
GTACCCC659.4828004E-426.1280737
CAGAGTA54150.025.52886410
ATACGTA1209.9291356E-825.46952211
Produced by FastQC (version 0.11.5)