FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126963
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40260.35724331677260035No Hit
GTAAAGACCTTCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAG38000.3371894197058821No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37050.32875968421323504No Hit
CAGTAAAGACCTTCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAGCGAACTGTGGCTGCACCAT35610.31598197988753846No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTACATAGTGATGGGAAGACCTATTTGT27930.2478342234838233No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27150.24091296697407102No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26840.23816221118173356No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24050.2134054090506964No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22890.2031122583438853No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22100.19610226777631565No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAGCGAACTGTGGCTGCACCA21750.19299657575270882No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21700.19255290546362214No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21310.189092277208746No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20780.1843893721444271No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20630.18305836127716704No Hit
CCTACATAGTGATGGGAAGACCTATTTGTCTTGGTATCTGCAGAAGCCAG20630.18305836127716704No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20590.18270342504589768No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20270.1798639351957429No Hit
GTCTTTACTGTGCATGCAATAATAAATCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT19760.1753384982470587No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGG19180.17019192289365312No Hit
CTCCTACATAGTGATGGGAAGACCTATTTGTCTTGGTATCTGCAGAAGCCAGGCCAGCCTCCACAACTCCTGAT18990.16850597579512372No Hit
GTGATGGGAAGACCTATTTGTCTTGGTATCTGCAGAAGCCAGGCCAGCCTCCACAACTCCTGATCTATGAAGTTT18620.16522281565588223No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18340.1627382620369968No Hit
CAGTAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA18040.16007624030247664No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17020.15102536640510825No Hit
GGATACCTGGATCCAGTGCAGATATTGTGATGGCCCAAACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG16700.14818587655495347No Hit
ATGTAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAG16570.14703233380332806No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA15830.14046601352484508No Hit
GTAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15750.13975614106230638No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGGCCCAAACTCCACTCTCT15630.13869133236849834No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15530.13780399179032496No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA15510.13762652367469028No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15280.13558564034489154No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15250.13531943817143952No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTGTACGGAAGGT15180.13469829976671815No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14940.13256868237910205No Hit
GGCCAAAAGTGTACGGAAGGTCTTTACTGTGCATGCAATAATAAATCCCA14790.131237671511842No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14470.1283981816616872No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14240.12635729833188847No Hit
CCTGGATCCAGTGCAGATATTGTGATGGCCCAAACTCCACTCTCTCTGTC14170.1257361599271671No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14110.12520375558026306No Hit
GCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGGCCCAAACTCCACTCTCTCTGTCCGTCACCC13870.12307413819264697No Hit
GTGCAGATATTGTGATGGCCCAAACTCCACTCTCTCTGTCCGTCACCCCT13850.12289667007701228No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG13810.12254173384574293No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13670.12129945703630021No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT13650.12112198892066554No Hit
GTATTACTGTGCGAGAATGGATCCCTGGGCCGTTTTTAATATGTGGGGCC13500.11979097805340548No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC13190.11704022226106801No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGAT12700.11269225342801847No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12500.11091757227167175No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12430.11029643386695039No Hit
GGTCTTTACTGTGCATGCAATAATAAATCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT12380.1098527635778637No Hit
GTTCTCTGGAGTGCCGGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATT12310.10923162517314233No Hit
CTCCTGATCTATGAAGTTTCCAAGGGGTTCTCTGGAGTGCCGGATAGGTT12090.10727947590116092No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12080.10719074184334357No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTTTGGGCCATCACAATATCT12050.10692453966989156No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG11870.10532732662917949No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11860.10523859257136214No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11840.10506112445572748No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11740.1041737838775541No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11710.1039075817041021No Hit
TCTCTGGAGTGCCGGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGG11680.10364137953065007No Hit
AGTCTAGTCAGAGCCTCCTACATAGTGATGGGAAGACCTATTTGTCTTGGTATCTGCAGAAGCCAGGCCAGCCTC11640.10328644329938073No Hit
CTATGTAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCC11640.10328644329938073No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11420.1013342940273993No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGGCCCAAACTCCACT11330.10053568750704325No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11330.10053568750704325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG100.008388951137.0654870
CTACGAT200.00792407150.79055814
GGTATCA8050.042.905731
GTATCAA22750.039.741191
TCGTATG2800.039.33999640
CGTATGC2850.037.51139541
TCTCCGA4200.037.0851711
ACGAGAC4150.035.90812322
ATCTCGT3250.035.85594637
CCCACGA4250.035.84738519
GATCTCG3250.035.80608736
GTGGTAT1850.034.777061
TACACAT4600.034.591865
CGAGACA4450.034.25602723
ATCTCCG4550.034.23094610
ATCAACG26200.034.1143073
GTATAAT502.0771772E-433.8618741
TTATACA4700.033.8558652
TATACAC4700.033.8558653
ACACATC4950.033.513896
Produced by FastQC (version 0.11.5)