FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126963
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44970.39903705800456624No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40700.3611476153165632No Hit
GTAAAGACCTTCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAG33770.2996549132491484No Hit
CAGTAAAGACCTTCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAGCGAACTGTGGCTGCACCAT31790.28208556980131555No Hit
GTCTTTACTGTGCATGCAATAATAAATCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT30360.2693965995334363No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29920.26549230098947346No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27580.24472853146021656No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27410.24322005247732179No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21740.1929078416948915No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAGCGAACTGTGGCTGCACCA21560.19131062865417942No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21290.1889148090931113No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTACATAGTGATGGGAAGACCTATTTGT21200.18811620257275527No Hit
GGTCTTTACTGTGCATGCAATAATAAATCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT19950.1770244453455881No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19930.1768469772299534No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19750.17524976418924135No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19170.17010318883583578No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19020.16877217796857572No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18750.1663763584075076No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18500.1641580069620742No Hit
GGCCAAAAGTGTACGGAAGGTCTTTACTGTGCATGCAATAATAAATCCCA17730.1573254845101392No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGG16980.1506704301738389No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16860.14960562148003084No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16680.14800840843931878No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15990.1418857584499225No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15890.1409984178717491No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15660.13895753454195037No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTGTACGGAAGGT15640.13878006642631568No Hit
ATGTAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAG15590.138336396137229No Hit
GTAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15480.13736032150123828No Hit
GGATACCTGGATCCAGTGCAGATATTGTGATGGCCCAAACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG15270.1354969062870742No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15030.1333672888994581No Hit
CAGTAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA14950.13265741643691942No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA14840.1316813418009287No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14680.1302615968758513No Hit
CTCCTACATAGTGATGGGAAGACCTATTTGTCTTGGTATCTGCAGAAGCCAGGCCAGCCTCCACAACTCCTGAT14590.12946299035549524No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG14440.12813197948823518No Hit
GTGATGGGAAGACCTATTTGTCTTGGTATCTGCAGAAGCCAGGCCAGCCTCCACAACTCCTGATCTATGAAGTTT14160.12564742586934974No Hit
CCTACATAGTGATGGGAAGACCTATTTGTCTTGGTATCTGCAGAAGCCAG14050.12467135123335904No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13930.12360654253955097No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13540.12014591428467483No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGGCCCAAACTCCACTCTCT13500.11979097805340548No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13380.11872616935959743No Hit
GTATTACTGTGCGAGAATGGATCCCTGGGCCGTTTTTAATATGTGGGGCC13020.11553174327817328No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12900.11446693458436524No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC12730.11295845560147051No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12400.11003023169349838No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12400.11003023169349838No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12330.10940909328877702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12230.10852175271060364No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA12080.10719074184334357No Hit
CCTGGATCCAGTGCAGATATTGTGATGGCCCAAACTCCACTCTCTCTGTC12070.10710200778552624No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12020.10665833749643955No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11980.1063034012651702No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTTTGGGCCATCACAATATCT11950.10603719909171819No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG11880.10541606068699683No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGAT11670.10355264547283274No Hit
GGTGGAGGCTGAGGATGTTGGGATTTATTATTGCATGCACAGTAAAGACCTTCCGTACACTTTTGGCCAGGGGAC11490.10195543243212066No Hit
GCTAATGCTCTGGATACCTGGATCCAGTGCAGATATTGTGATGGCCCAAACTCCACTCTCTCTGTCCGTCACCC11320.10044695344922593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)