FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083975
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45460.4193823658294703No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43060.3972416338015176No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32840.30295901658248575No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32210.2971470744251482No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT31060.2865379736617542No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA30350.2799880071034848No Hit
GTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT29920.27602112594847666No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29770.2746373301967296No Hit
GGTCTAGTCAGACCCTCCTGCATAGTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTC29700.2739915588459143No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGACCCTCCTG29180.2691944002398579No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA27950.2578472750755322No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24270.2238981526326714No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24200.22325238128185612No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23820.21974676537743026No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23730.21891648792638205No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23560.2173481860744021No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22990.21208976221776332No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22480.20738485666182338No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21770.20083489010355404No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21360.1970525150487788No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAAAC21080.19446942964551764No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT20710.1910560667912083No Hit
GTGTATTACTGTGCGAGAGATCACAATTCGGATTTGATGATGACACTTGC19920.18376807583200722No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG19660.181369496528979No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT19610.18090823127839664No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19020.17546530132152494No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTATTCGGGCC18980.17509628912105907No Hit
GTCAGGGACCCCGGAGGCCCGAATAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTG18840.1738047464194285No Hit
GCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC18670.1722364445674485No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA18310.1689153347632556No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGA18190.167808298161858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18070.16670126156046033No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17160.15830623399986163No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCC17130.1580294748495122No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATTTCC17040.157199197398464No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA16680.1538780875942711No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16640.1535090753938052No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16620.15332456929357227No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16430.15157176134135936No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16000.14760488018635115No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15860.14631333748472058No Hit
ATGCAGGAGGGTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA15820.14594432528425472No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15740.14520630088332295No Hit
GTTTATTACTGCATGCAAACTCTACAAACCTCCACTTTTGGCCAGGGGAC15720.14502179478309002No Hit
CTCTACAAACCTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA15640.14428377038215826No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15560.14354574598122652No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15300.1411471666781983No Hit
TTATTACTGCATGCAAACTCTACAAACCTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG15210.14031688922715008No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG15140.13967111787633477No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTATTCGGGCCTCCGGGGTCCCT14770.13625775502202542No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14600.13468945317004544No Hit
AGTCAGACCCTCCTGCATAGTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAA14410.13293664521783252No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14410.13293664521783252No Hit
CTACAAACCTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT14340.13229087386701724No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGACTCAGTCTCCACT14260.13155284946608548No Hit
GGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT14180.1308148250651537No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13960.12878525796259138No Hit
GCCCTGGCTTCTGCAGGTGCCACTCCAAATAGTTGTATCCATTACTATGC13840.12767822136119375No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13650.12592541340898084No Hit
CAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTATTCGGGC13620.12564865425863142No Hit
GGCCAAAAGTGGAGGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACA13440.12398809935653499No Hit
GCATAGTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGC13420.12380359325630205No Hit
ATCCATTACTATGCAGGAGGGTCTGACTAGACCTGCAGGAGATGGAGGCC13350.12315782190548674No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13190.12168177310362324No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13180.12158952005350677No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13100.120851495652575No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTGCCACTCCAAATAGTTGTATCCATTACTATGCAGGAGGGTCTGA13020.12011347125164326No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTATTCGGGCCTCCGGGGTCCCTGACAGGTT12980.11974445905117738No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA12740.1175303858483821No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12620.11642334924698448No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG12490.11522405959547037No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12390.11430152909430569No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12370.11411702299407274No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAA12230.11282548029244216No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11880.10959662353836573No Hit
GGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTATT11880.10959662353836573No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11820.10904310523766692No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11750.10839733388685163No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGACTCAGTCTC11630.10729029728545399No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCACCACCTACGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGA11580.10682903203487165No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11580.10682903203487165No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11460.10572199543347402No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11410.10526073018289168No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11360.10479946493230934No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11270.10396918748126109No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11250.10378468138102816No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11210.10341566918056228No Hit
GAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATG11080.10221637952904819No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11060.10203187342881526No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10860.10018681242648586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)