FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083975
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46770.4314675153947277No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37780.3485320233400217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34410.31744274545077145No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32160.29668580917456583No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA31660.2920731566687424No Hit
GGTCTAGTCAGACCCTCCTGCATAGTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTC31200.2878295163633848No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT31100.2869069858622201No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGACCCTCCTG29450.2716852325930026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28930.2668880739869462No Hit
GTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT28860.2662423026361309No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28650.26430498858368506No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25340.23376922899513367No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24910.22980234784012546No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24800.2287875642888443No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA24130.22260660993104084No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23060.2127355335685786No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21950.20249544500565048No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21510.19843631080052582No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21380.19723702114901173No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAAAC20410.1882884752877142No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20280.1870891856362001No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT20040.18487511243340482No Hit
GTGTATTACTGTGCGAGAGATCACAATTCGGATTTGATGATGACACTTGC19890.18349131668165777No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG19090.17611107267234025No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTATTCGGGCC18950.17481952997070965No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18550.1711294079660509No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18420.16993011831453678No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT18250.1683618164625568No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18180.1677160451117415No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCC18000.16605549020964505No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGA17780.16402592310708272No Hit
GTCAGGGACCCCGGAGGCCCGAATAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTG17630.1626421273553357No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17490.1613505846537051No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17440.16088931940312276No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATTTCC17210.15876749925044395No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17060.15738370349869693No Hit
GCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC16930.15618441384718282No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA16680.1538780875942711No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA16560.15277105099287344No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA16090.1484351576373994No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15670.14456052953250767No Hit
GTTTATTACTGCATGCAAACTCTACAAACCTCCACTTTTGGCCAGGGGAC15480.14280772158029473No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTATTCGGGCCTCCGGGGTCCCT15450.14253096242994534No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG15420.14225420327959593No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15330.1414239258285477No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15330.1414239258285477No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14980.13819506907447127No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14760.13616550197190894No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14660.13524297147074427No Hit
CTCTACAAACCTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14560.13432044096957954No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGACTCAGTCTCCACT14530.13404368181923015No Hit
TTATTACTGCATGCAAACTCTACAAACCTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG14330.13219862081690076No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14300.13192186166655134No Hit
GGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT14300.13192186166655134No Hit
GCCCTGGCTTCTGCAGGTGCCACTCCAAATAGTTGTATCCATTACTATGC14200.13099933116538665No Hit
ATGCAGGAGGGTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA14130.13035355981457136No Hit
AGTCAGACCCTCCTGCATAGTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAA13930.12850849881224197No Hit
CTACAAACCTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT13800.12730920916072788No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13720.12657118475979612No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13680.12620217255933022No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTGCCACTCCAAATAGTTGTATCCATTACTATGCAGGAGGGTCTGA13640.12583316035886435No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13060.12048248345210913No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13050.12039023040199266No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13030.12020572430175973No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13010.12002121820152678No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12930.11928319380059503No Hit
CAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTATTCGGGC12780.11789939804884798No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12620.11642334924698448No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12560.11586983094628567No Hit
ATCCATTACTATGCAGGAGGGTCTGACTAGACCTGCAGGAGATGGAGGCC12460.11494730044512096No Hit
GGCCAAAAGTGGAGGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACA12010.11079591318987984No Hit
GGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTATT11980.11051915403953044No Hit
GAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATG11900.10978112963859867No Hit
GCATAGTAATGGATACAACTATTTGGAGTGGCACCTGCAGAAGCCAGGGC11900.10978112963859867No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11810.10895085218755045No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTATTCGGGCCTCCGGGGTCCCTGACAGGTT11710.10802832168638576No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAA11670.10765930948591988No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCACCACCTACGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGA11660.10756705643580342No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11590.10692128508498812No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11590.10692128508498812No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11520.10627551373417284No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11470.10581424848359049No Hit
CTCCTGATCTATTTGGGTTCTATTCGGGCCTCCGGGGTCCCTGACAGGTT11450.10562974238335755No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG11450.10562974238335755No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11430.10544523628312462No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11370.1048917179824258No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCGGTGGGGAAATTGTGATGACTCAGTCTC11350.10470721188219284No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11040.1018473673285823No Hit
GGGTCTCTGGATCCGGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG10930.10083258377730113No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10880.1003713185267188No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10880.1003713185267188No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10850.10009455937636938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA250.006089208654.28735469
TCTGGAG10000.049.3687270
TCTATCG450.00903858349.12310470
CGGGCCA750.001369143439.29848570
GTTATTA959.345491E-538.78140370
ACGATTC350.001484629738.66036614
ACAGGTA1350.035.083941
GTGTTGC502.0883053E-433.830941
GACAGCC2200.033.830941
GCTTGCA906.2827894E-933.830941
GTATCAA18950.032.849041
ATTGTCT9850.032.79614364
AATTGTC10150.032.08431663
GCTTGTA951.0644726E-832.0503651
TGTTAGA12500.031.24229670
GCTTATC553.6531317E-430.7554021
CGGAATT10500.030.72364660
CGAAACC1052.780258E-630.15964169
CGTCTAT450.00508719930.0719471
GCTTGAT902.3685425E-730.0719471
Produced by FastQC (version 0.11.5)