FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004689555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881889
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76430.4061344744562511No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68080.36176416356118773No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52050.27658379426204205No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46050.24470093613385271No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45290.24066244077094878No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44720.23763356924877077No Hit
CTACAAACTCCTCGGACTTTTGGACAGGGGACCAATCTAGAGATCAAACG43040.22870636897287777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40750.21653774478728555No Hit
GATTTTACACTGATAATCAGCAGAGTGGAGGCTGAGGATGTTGGTGTTTATTACTGCATGCAAGCTCTACAAACT40520.2153155685590383No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG39550.21016117316164767No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38800.20617581589562403No Hit
GTCCAAAAGTCCGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACACCA38720.2057507111205815No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38470.2044222586985736No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTC37830.20102142049823343No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36640.19469798696947588No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34860.18523940572477973No Hit
GGTCTAGTCAGAGCCTCCTCCATAGTAATAGATACAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGCCTC34330.18242308659012302No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT33730.17923480077730408No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT33700.17907538648666313No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32470.17253940057038433No Hit
GTAATAGATACAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGGT31780.16887287188564257No Hit
CTTTTGGACAGGGGACCAATCTAGAGATCAAACGAACTGTGGCTGCACCA31690.1683946290137197No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30930.16435613365081575No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30850.16393102887577324No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGACAGGGGACCAATCTAGAGATCAAACGAA30570.16244316216312438No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGATTGGTCC30230.16063646686919367No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30110.15999880970662989No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAT29680.15771387154077632No Hit
GTAATAAACACCAACATCCTCAGCCTCCACTCTGCTGATTATCAGTGTAAAATCTGTGCCTGATCCACTGCCA29630.15744818105637473No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACACCAACATCCTCAGCCTCCACTCTGCTGATTATCAGTGTAAAAT29520.15686366199069127No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGATTGGTCCCCTGTCCAAAAGTC29410.1562791429250078No Hit
GATAATCAGCAGAGTGGAGGCTGAGGATGTTGGTGTTTATTACTGCATGC28890.1535159618872314No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG28630.15213437136834318No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27080.14389796635189428No Hit
ATTTGGGGTCCAGTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT26570.1411879234109982No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26240.13943436621394778No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26030.13831846617946117No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25900.13762767092001707No Hit
AGCCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGGTCCAGTCGGGCC25470.1353427327541635No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24970.13268582791014774No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGATAATCAGCAGAG24770.13162306597254142No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT24720.13135737548813983No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24670.13109168500373825No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC24360.1294444040004485No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24280.12901929922540598No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA23900.12700005154395397No Hit
GTCAGGGACCCCGGAGGCCCGACTGGACCCCAAATAGATCAGGAGCTGTGGAGGCTGCCCTGGCTTCTGCACGTA23380.12423687050617757No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23360.12413059431241694No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22930.12184565614656337No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGACAGGGGA22920.12179251804968305No Hit
ATCTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG22820.12126113708087989No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC22460.11934816559318856No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG22350.11876364652750508No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC21640.11499084164900268No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21470.11408749400203733No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21100.11212138441746564No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21030.11174941773930344No Hit
CAGTAATAAACACCAACATCCTCAGCCTCCACTCTGCTGATTATCAGTGTAAAATCTGTGCCTGATCCACTGCCA20350.10813602715144198No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19930.10590422708246874No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19630.10431008417605926No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT19520.10372556511037578No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19310.10260966507588916No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA19300.10255652697900884No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19140.10170631742892382No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA19110.10154690313828287No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19010.1010155221694797No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18950.10069669358819783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)