FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881889
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87030.46246085714938556No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75870.4031587410309535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53430.2839168516315256No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51560.2739800275149066No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51150.27180136554281364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42740.22711222606646833No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG42140.22392394025364937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41400.21999172108450607No Hit
GTCCAAAAGTCCGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACACCA39110.2078230968989138No Hit
CTACAAACTCCTCGGACTTTTGGACAGGGGACCAATCTAGAGATCAAACG38060.20224359672648068No Hit
GATTTTACACTGATAATCAGCAGAGTGGAGGCTGAGGATGTTGGTGTTTATTACTGCATGCAAGCTCTACAAACT37850.20112769669199407No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35420.1882151391500774No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34930.18561137240294195No Hit
GGTCTAGTCAGAGCCTCCTCCATAGTAATAGATACAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGCCTC34080.1810946341681151No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACACCAACATCCTCAGCCTCCACTCTGCTGATTATCAGTGTAAAAT33690.1790222483897828No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33330.17710927690209147No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32130.1707327052764536No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32060.1703607385982914No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTC31890.16945739095132603No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT31480.1672787289792331No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31480.1672787289792331No Hit
GTAATAAACACCAACATCCTCAGCCTCCACTCTGCTGATTATCAGTGTAAAATCTGTGCCTGATCCACTGCCA31300.16632224323538744No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31000.16472810032897794No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30760.16345278600385038No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGACAGGGGACCAATCTAGAGATCAAACGAA30730.16329337171320943No Hit
GTAATAGATACAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGGT30520.16217747167872282No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29600.15728876676573378No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGATTGGTCCCCTGTCCAAAAGTC29570.15712935247509285No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGATTGGTCC29410.1562791429250078No Hit
CTTTTGGACAGGGGACCAATCTAGAGATCAAACGAACTGTGGCTGCACCA29080.15452558572795738No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28510.1514967142057794No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28190.1497962951056093No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27400.14559838545206438No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26130.13884984714826432No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAT25770.13693687566057297No Hit
ATTTGGGGTCCAGTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT24860.13210130884446428No Hit
GATAATCAGCAGAGTGGAGGCTGAGGATGTTGGTGTTTATTACTGCATGC24850.13204817074758396No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24670.13109168500373825No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGATAATCAGCAGAG24220.12870047064412407No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT24220.12870047064412407No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24060.12785026109403902No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23940.12721260393147524No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23700.12593728960634765No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC22980.12211134663096496No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC22700.12062347991831611No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22550.11982640846511139No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG22470.11940130369006885No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGACAGGGGA22300.1184979560431035No Hit
AGCCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGGTCCAGTCGGGCC22260.11828540365558222No Hit
GTCAGGGACCCCGGAGGCCCGACTGGACCCCAAATAGATCAGGAGCTGTGGAGGCTGCCCTGGCTTCTGCACGTA22140.11764774649301844No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC21870.11621301787724994No Hit
ATCTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG21700.11530967023028459No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21660.11509711784276332No Hit
CAGTAATAAACACCAACATCCTCAGCCTCCACTCTGCTGATTATCAGTGTAAAATCTGTGCCTGATCCACTGCCA21590.11472515116460111No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21370.11355611303323417No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21220.11275904158002943No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA20790.11047410341417586No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA20750.1102615510266546No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20520.10903937479840733No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20420.10850799382960419No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20070.10664816043879313No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20040.10648874614815221No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19320.10266280317276948No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT19240.10223769839772696No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19220.10213142220396633No Hit
GAGTGGAGGCTGAGGATGTTGGTGTTTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGACAGGGGA19070.10133435075076161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)