FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076745
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48860.45377503494327814No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT45460.4221983849472252No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44740.4155115649480611No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32840.30499328996187586No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT32020.29737774496282776No Hit
TTATAATAGTTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT31160.28939070996382615No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29610.27499547246562556No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCAGATGATTTTGCAACTTATTACTGCCAACATTATAATAGTTAC29550.27443823746569523No Hit
CTATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCTGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC28770.2671941824666007No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28760.26710130996661235No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28210.2619933224672509No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26570.2467622324691547No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26230.24360456746954942No Hit
CATTATAATAGTTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGA26030.2417471174697816No Hit
CCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG25640.23812508997023435No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25500.2368248749703969No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25430.23617476747047816No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24400.22660889997167388No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24310.22577304747177837No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24220.22493719497188283No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23720.2202935699724633No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23400.21732164997283482No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23060.21416398497322947No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20270.18825255747646844No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTA20100.1866737249766658No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT20050.18620936247672384No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19270.17896530747762934No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA18930.17580764247802405No Hit
ATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCTGGCTGCAGGCTGC18850.17506466247811692No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18740.17404306497824462No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18530.1720927424784884No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA17400.16159814997980024No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17280.16048367997993954No Hit
GTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17200.1597406999800324No Hit
GGCCAAAAGTGTACATGAGGTAACTATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCTGGCTGCAGGCTG17180.15955495498005565No Hit
CTTCTAAAGTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAG16930.15723314248034587No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16840.15639728998045033No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16700.15509707498061287No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16590.15407547748074055No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16530.15351824248081022No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15970.14831738248146034No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15810.14683142248164607No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15460.14358088498205238No Hit
GTCTCCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG15400.14302364998212205No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA15190.14107332748236584No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14760.13707980998286504No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14600.13559384998305077No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA14190.13178607748352675No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14150.13141458748357318No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13900.1290927749838634No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13840.12853553998393308No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13740.12760681498404916No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13730.12751394248406075No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13560.1259351099842581No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13420.12463489498442062No Hit
TCTATAAGGCGTCTACTTTAGAAGTTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACT13400.12444914998444385No Hit
CTTTAGAAGTTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGC13400.12444914998444385No Hit
CATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG13390.12435627748445548No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13320.12370616998453672No Hit
CCCCTAAACTCCTGATCTATAAGGCGTCTACTTTAGAAGTTGGGGTCCCA12890.1197126524850359No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA12790.118783927485152No Hit
ACTTATTACTGCCAACATTATAATAGTTACCTCATGTACACTTTTGGCCA12680.11776232998527972No Hit
GTGTACATGAGGTAACTATTATAATGTTGGCAGTAATAAGTTGCAAAATC12440.11553338998555832No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12380.11497615498562799No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12230.11358306748580213No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12210.11339732248582532No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12050.11191136248601108No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12000.11144699998606913No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11990.11135412748608073No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11890.11042540248619682No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11870.11023965748622004No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11870.11023965748622004No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCAGATGATT11720.10884656998639418No Hit
TAGTTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT11590.1076392274865451No Hit
CTCCTGATCTATAAGGCGTCTACTTTAGAAGTTGGGGTCCCATCAAGGTT11540.10717486498660314No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11490.10671050248666118No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCAGATGATTTTG11470.10652475748668441No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11350.10541028748682373No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11270.10466730748691659No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTACTTTAGAAGTTGGGGTCCCAT11230.10429581748696301No Hit
ACCTTGATGGGACCCCAACTTCTAAAGTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG10910.10132389748733452No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10860.10085953498739256No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10800.10030229998746222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.00397209460.47158470
CGCGTAT453.1188392E-853.4659139
ATCGCGT451.9609633E-645.6527337
ATATCGT405.230483E-542.7994337
AATCGCG504.099442E-641.01196736
ATGCCGT6900.040.83017344
GTGGTAT2900.039.5125921
CGAGACT7950.039.0263523
CGTATGC7100.038.91403641
CTTATAC8050.038.516481
AATCTCG6750.038.48036236
TATACAC8600.038.3902443
TATCGTA451.034183E-438.12491238
ACGAGAC8250.038.01067722
AATATCG451.0586128E-437.97404536
CACGAGA8350.037.95575321
GCTAATC7400.037.72036433
TCGTATG6950.037.6693740
ATCTCGT6550.037.63736337
CCCACGA8400.037.3145719
Produced by FastQC (version 0.11.5)