FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076745
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75480.7010016299123748No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47310.4393797974450775No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT41890.38904290245136963No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33780.3137233049607846No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32000.297191999962851No Hit
TTATAATAGTTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT31910.29635614746295547No Hit
CTATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCTGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC31440.2919911399635011No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31380.29143390496357074No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCAGATGATTTTGCAACTTATTACTGCCAACATTATAATAGTTAC27380.25428490496821443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25490.23673200247040851No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25450.23636051247045492No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25440.23626763997046654No Hit
CATTATAATAGTTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGA24440.22698038997162745No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24280.22549442997181318No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23580.21899335497262581No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23120.21472121997315985No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23000.21360674997329918No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA21820.20264779497466903No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21440.19911863997511017No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20700.19224607497596924No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20650.1917817124760273No Hit
CCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG20350.18899553747637557No Hit
ATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCTGGCTGCAGGCTGC19980.1855592549768051No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19620.182215844977223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19000.17645774997794278No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18950.1759933874780008No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18860.17515753497810532No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTA18130.16837784247895277No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA18110.16819209747897598No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17980.1669847549791269No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17640.1638270899795216No Hit
CTTCTAAAGTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAG17590.16336272747957964No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17570.16317698247960288No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT17420.16178389497977702No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17150.15927633748009046No Hit
GGCCAAAAGTGTACATGAGGTAACTATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCTGGCTGCAGGCTG16890.1568616524803923No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16810.15611867248048517No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16370.15203228248099596No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16180.15026770498121655No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAACTTCTAAAGT15420.14320939498209884No Hit
GTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA14820.1376370449827954No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14820.1376370449827954No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14630.13587246748301593No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA14450.1342007624832249No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14300.13280767498339904No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14230.13215756748348031No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14210.13197182248350353No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14090.13085735248364283No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA14050.13048586248368926No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13810.1282569224839679No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA13690.12714245248410722No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13560.1259351099842581No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13420.12463489498442062No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13410.12454202248443225No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13240.12296318998462959No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13130.1219415924847573No Hit
GTGTACATGAGGTAACTATTATAATGTTGGCAGTAATAAGTTGCAAAATC13050.12119861248485017No Hit
ACTTATTACTGCCAACATTATAATAGTTACCTCATGTACACTTTTGGCCA12850.11934116248508235No Hit
GTCTCCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG12760.11850530998518685No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG12550.11655498748543063No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12550.11655498748543063No Hit
TCTATAAGGCGTCTACTTTAGAAGTTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACT12460.1157191349855351No Hit
CTTTAGAAGTTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGC12270.11395455748575568No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG12090.11228285248596465No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12010.11153987248605751No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11950.11098263748612718No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11780.10940380498632453No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCAGATGATT11710.10875369748640579No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11670.10838220748645222No Hit
TAGTTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT11650.10819646248647545No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11610.10782497248652187No Hit
GTAATAAGTTGCAAAATCATCTGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCG11550.10726773748659155No Hit
ACCTTGATGGGACCCCAACTTCTAAAGTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG11450.10633901248670763No Hit
CATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG11130.1033670924870791No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11120.10327421998709073No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11060.10271698498716038No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10770.10002368248749705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)