FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944455
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATAGTTACCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT39570.41897178796236983No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC37310.39504264364104164No Hit
GTAATACACAGCCGTGTCCTCGCCTCGCAGGCTGTTCATTTGCAGATAAA31000.3282316256465369No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30530.3232552106770571No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29100.3081142034294911No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATTAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA25640.27147931876055503No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20450.21652699175715098No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20330.21525641772239018No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18600.19693897538792215No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18100.1916449169097522No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17990.1904802240445548No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17460.18486852205769466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16610.17586862264480574No Hit
CAATATAATAGTTACCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT16320.17279806872746717No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15990.169303990131875No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15830.16760989141886062No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATCATTTAGCTTGGTTTCAGCAGAAG15680.16602167387540961No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15580.16496286217977563No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC15560.16475109984064884No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15510.16422169399283185No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15460.16369228814501485No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15160.16051585305811286No Hit
CCATAGATCAGGGACTTAGGGGCTTTCCCTGGCTTCTGCTGAAACCAAGC14890.1576570614799011No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA14730.15596296276688673No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13920.1473865880322514No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13880.1469630633539978No Hit
GTGTATTACTGTGCGAGTTTTGCTGATTACTGGGGCCAGGGAACCCTGGT13860.14675130101487102No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13030.13796316394110889No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG12650.13393967949769972No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATTAAAGTTCAGCGGCAGTGGA12640.13383379832813633No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12330.13055148207167097No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT12190.12906914569778336No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11840.1253633047630644No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11750.12441037423699382No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11560.12239863201528924No Hit
GGCCAAAAGTGTGAGGGTAACTATTATATTGTTGGCAGTAATAAGTTGCA11530.12208098850659903No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT11440.12112805798052845No Hit
CAGTAATACACAGCCGTGTCCTCGCCTCGCAGGCTGTTCATTTGCAGATA11360.12028100862402126No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAATATAATAGTTAC11080.11731633587624608No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT10990.1163634053501755No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10960.11604576184148531No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10920.11562223716323171No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC10840.11477518780672452No Hit
CTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG10650.11276344558501994No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10650.11276344558501994No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA10420.11032817868506177No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10350.10958701049811796No Hit
GGGCATTAGCAATCATTTAGCTTGGTTTCAGCAGAAGCCAGGGAAAGCCCCTAAGTCCCTGATCTATGGTGCATC10340.10948112932855456No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10320.10926936698942777No Hit
GTGGATGCACCATAGATCAGGGACTTAGGGGCTTTCCCTGGCTTCTGCTGAAACCAAGCTAAATGATTGCTAATG10220.10821055529379377No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCGGGGGGGTCCCTGAGACTCTCCTGTGAAGC10000.105881169563399No Hit
GTCCCTGATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATTAAAGT9870.10450471435907481No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9840.10418707085038462No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA9770.10344590266344082No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9630.10196356628955323No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC9570.10132827927217285No Hit
AGTCAGGGCATTAGCAATCATTTAGCTTGGTTTCAGCAGAAGCCAGGGAA9530.10090475459391925No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9520.10079887342435585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)