FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944455
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATAGTTACCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT38580.40848955217559335No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC37100.39281913908021027No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31130.3296080808508611No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27600.29223202799498127No Hit
GTAATACACAGCCGTGTCCTCGCCTCGCAGGCTGTTCATTTGCAGATAAA26980.2856673954820505No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATTAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA23970.2537971634434674No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23900.25305599525652356No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21120.2236210301178987No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20170.21356231900937578No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19690.20848002287033263No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16690.1767156720013129No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16680.17660979083174955No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15490.16400993165370503No Hit
CAATATAATAGTTACCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT15470.16379816931457825No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15460.16369228814501485No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15090.15977468487116908No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14900.1577629426494645No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14680.1554335569190697No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14460.15310417118867495No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA14140.14971597376264617No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14000.14823363738875858No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC13830.14643365750618081No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATCATTTAGCTTGGTTTCAGCAGAAG13780.14590425165836382No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13600.14399839060622263No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13540.14336310358884224No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13420.14209252955408144No Hit
CCATAGATCAGGGACTTAGGGGCTTTCCCTGGCTTCTGCTGAAACCAAGC12840.13595142171940433No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC12550.13288086780206573No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12220.12938678920647356No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11930.126316235289135No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG11840.1253633047630644No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11800.12493978008481081No Hit
GTGTATTACTGTGCGAGTTTTGCTGATTACTGGGGCCAGGGAACCCTGGT11620.12303391903266962No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11350.12017512745445785No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11080.11731633587624608No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11060.11710457353711928No Hit
GGCCAAAAGTGTGAGGGTAACTATTATATTGTTGGCAGTAATAAGTTGCA11020.11668104885886571No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATTAAAGTTCAGCGGCAGTGGA10900.11541047482410492No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAATATAATAGTTAC10810.11445754429803433No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCGGGGGGGTCCCTGAGACTCTCCTGTGAAGC10770.11403401961978073No Hit
GTGGATGCACCATAGATCAGGGACTTAGGGGCTTTCCCTGGCTTCTGCTGAAACCAAGCTAAATGATTGCTAATG10530.11149287155025915No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT10360.10969289166768137No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTAATGGGACCCCA10120.1071517435981598No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10050.106410575411216No Hit
GTCCCTGATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATTAAAGT10010.1059870507329624No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9850.10429295201994802No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9830.10408118968082121No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT9820.10397530851125783No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9790.10365766500256762No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9770.10344590266344082No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9680.10249297213737023No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9660.10228120979824343No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT9580.10143416044173625No Hit
CAGTAATACACAGCCGTGTCCTCGCCTCGCAGGCTGTTCATTTGCAGATA9580.10143416044173625No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG9510.10069299225479245No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA9490.10048122991566566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA150.00244922268.3237320
GGCGTCG200.007319404351.8199541
TCTGGAG10150.046.38201570
GACAGGT2100.043.8712121
GGTATCA10050.042.4403651
GTGGTAT2650.039.9163671
GCATCCG5750.034.21614570
GACACCG400.00273732734.1292847
TGGTATC2900.034.1202432
AATGGTA2208.6765795E-1031.93877470
TCAACGC27950.031.7498574
ATCAACG28000.031.688123
CAACGCA28350.031.179845
AACGCAG28700.030.5617646
ACTGAGT450.00487308930.3371438
TATACTG450.00487308930.3371435
AAAACCG950.00550621129.5853970
CGCAGAG30100.029.3670588
GTATCAA32750.028.9646741
ACGCAGA30350.028.9002517
Produced by FastQC (version 0.11.5)