FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294578
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40180.3103714106063906No Hit
GTAATATACGGCCGTGTCCTCGGGTCTCAGGCTGTTCATCTGCAGATACA39180.3026468857032948No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37600.29044213635640337No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG32370.25004287111321216No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27130.20956636062099002No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26200.2023825524611109No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25580.19759334702119147No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24640.19033229361228138No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23350.18036765648728775No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23050.178050299016359No Hit
ATACTATTGTCAACAGTATGATAAAATCTGGGTTTTCGGCGGAGGGACCAAGGTGGAGATCAATCGAACTGTGG22840.17642814878670887No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAAGACATTAATAGTTCTTTAAATTGGTATCAGCAGAAA22440.17333833882547053No Hit
GTATATTACTGTGCGAGACGCAAAGTGACTTCGTCTTCCTACTGGGGCCA21800.1683946428874892No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21110.16306472070435307No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21030.1624467587121054No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21010.1622922682140435No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20720.1600521559921457No Hit
GGACTGAACAGAGAGAACTCACCATGGAGATTGGGCTGAGCTGGCTTTTT20500.1583527605134646No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19570.1511689523535855No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18880.14583903017044936No Hit
CATTAATAGTTCTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTA18880.14583903017044936No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT18750.1448348419330469No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18700.1444486156878921No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18520.14305820120533486No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATATTGCAACATACTATTGTCAACAGTATGATAAAAT17890.1381917505163845No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATCTCCACCTTGGTCC17810.13757378852413682No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16980.13116243285456727No Hit
CAATAGTATGTTGCAATATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTCCCA16740.12930854687782428No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15820.12220198396697612No Hit
GAGTGAAATCTGTCCCAGATCCACTCCCACTGAACCTCGATGGGACCCCT15820.12220198396697612No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCCTGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG15610.12057983373732599No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15240.11772175952318052No Hit
CTATTAATGTCTTGACTCGCCTGACAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA15160.11710379753093285No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15010.1159451187954685No Hit
GTATAACTAGTGGCTGCGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCG14840.11463194956194218No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14720.11370500657357069No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATCTGACAT14700.11355051607550878No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14650.11316428983035398No Hit
CTAGTTATACTGGTCGTAGCACATATTATGCAGACTCCGTGAAGGGCCGG14630.11300979933229208No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14590.11270081833616823No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14400.11123315860458002No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14300.11046070611427045No Hit
GGTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAATTTAAAGAACTATTA14100.10891580113365126No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTCCCACTGAACCTCG13870.10713916040593922No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13570.10482180293501049No Hit
CAGTAATATACGGCCGTGTCCTCGGGTCTCAGGCTGTTCATCTGCAGATA13500.10428108619179377No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATCTGACATCCAGATGACCCAGTCTCCATCCTCC13400.10350863370148419No Hit
ATTGTCAACAGTATGATAAAATCTGGGTTTTCGGCGGAGGGACCAAGGTGGAGATCAATCGAACTGTGGCTGCAC13380.10335414320342226No Hit
GTATGATAAAATCTGGGTTTTCGGCGGAGGGACCAAGGTGGAGATCAATCGAACTGTGGCTGCACCATCTGTCTT13290.10265893596214365No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13190.10188648347183406No Hit
GTTGACAATAGTATGTTGCAATATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCA13120.10134576672861735No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13010.10049606898927682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)