FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294578
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63220.48834446437371865No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40570.313383975318598No Hit
GTAATATACGGCCGTGTCCTCGGGTCTCAGGCTGTTCATCTGCAGATACA36770.28403078068683385No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG31420.24270457245527113No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31320.24193211996496158No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27600.21319688732544506No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27280.2107250393564544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22960.17735509177508038No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22810.176196413039616No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22110.1707892456074489No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAAGACATTAATAGTTCTTTAAATTGGTATCAGCAGAAA21930.16939883112489165No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21920.16932158587586071No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21550.16646351166171525No Hit
ATACTATTGTCAACAGTATGATAAAATCTGGGTTTTCGGCGGAGGGACCAAGGTGGAGATCAATCGAACTGTGG20980.16206053246695062No Hit
GTATATTACTGTGCGAGACGCAAAGTGACTTCGTCTTCCTACTGGGGCCA19940.15402702656773093No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19900.15371804557160712No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19580.15124619760261646No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19150.14792465189428525No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18750.1448348419330469No Hit
GGACTGAACAGAGAGAACTCACCATGGAGATTGGGCTGAGCTGGCTTTTT18440.1424402392130872No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATATTGCAACATACTATTGTCAACAGTATGATAAAAT18140.14012288174215845No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18100.13981390074603461No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17360.1340977523177437No Hit
CATTAATAGTTCTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTA17330.1338660165706508No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATCTCCACCTTGGTCC17160.13255284733712452No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16680.1288450753836385No Hit
CAATAGTATGTTGCAATATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTCCCA16660.1286905848855766No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16490.12737741565205032No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16290.12583251067143114No Hit
GAGTGAAATCTGTCCCAGATCCACTCCCACTGAACCTCGATGGGACCCCT16240.12544628442627637No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCCTGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG15770.12181575772182132No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15630.1207343242353879No Hit
GGTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAATTTAAAGAACTATTA15020.11602236404449945No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14680.11339602557744685No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14520.11216010159295152No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATCTGACAT14350.11084693235942522No Hit
GTTGACAATAGTATGTTGCAATATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCA13860.10706191515690827No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13760.1062894626665987No Hit
CTATTAATGTCTTGACTCGCCTGACAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA13700.10582599117241294No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGAT13560.10474455768597954No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTCCCACTGAACCTCG13540.10459006718791759No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13540.10459006718791759No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13530.10451282193888664No Hit
GTATAACTAGTGGCTGCGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCG13480.10412659569373187No Hit
GTATGATAAAATCTGGGTTTTCGGCGGAGGGACCAAGGTGGAGATCAATCGAACTGTGGCTGCACCATCTGTCTT13400.10350863370148419No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13380.10335414320342226No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13360.10319965270536036No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13350.1031224074563294No Hit
CAGTAATATACGGCCGTGTCCTCGGGTCTCAGGCTGTTCATCTGCAGATA13310.10281342646020558No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12980.10026433324218392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTG150.002255554869.759348
CCGTTTA250.00636228253.68947269
GTGGTAT3100.043.8077161
GTATCAA36150.035.9701961
GACAGGT2850.034.546611
TATCAAC40100.032.0895772
TCTGGAG15950.031.07553570
ATCAACG41300.030.9093673
TCAACGC41600.030.6060334
AACGCAG41600.030.6024866
CAACGCA42100.030.3196745
ACGCAGA43500.029.2658277
GTGCTAG1750.029.1008431
CGCAGAG44100.029.0216128
ACGGGTT953.6611527E-728.61352216
AGAGTAC44700.028.551711
TTTTCCG605.8944395E-428.34286723
GTACGAC605.9561065E-428.2924863
ATACGTA1800.028.28592511
CAGAGTA45100.028.22648210
Produced by FastQC (version 0.11.5)