FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960099
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTAGTGGCTGGTTGGCCTGGTATCAGCAGAAAACAGGGAAAGCCCCTAGGCTCCTGATCTATAAGGCGT57940.6034794328501539No Hit
CTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC51360.5349448338140129No Hit
CTATAATAGTTATTCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGG46160.48078375250885586No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34540.3597545669769472No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34510.35944209920018666No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26330.27424255207015114No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25730.2679931965349407No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA25410.26466020691616177No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23480.24455811327790156No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA22580.2351840799750859No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21680.22581004667227025No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21560.22456017556522814No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20930.21799835225325723No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGG20750.2161235455926941No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20720.21581107781593356No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA20600.21456120670889145No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG20580.21435289485771783No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20120.20956172228072312No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19410.20216665156405747No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19330.20133340415936274No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19000.19789625861499702No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA18990.19779210268941017No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18480.1924801504844813No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18380.19143859122861287No Hit
GGCCAAAAGTGTACCCCGGAGAATAACTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG17850.18591832717251033No Hit
GTATTAGTGGCTGGTTGGCCTGGTATCAGCAGAAAACAGGGAAAGCCCCT17540.1826894934793183No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA16930.176335982018521No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCA16730.1742528635067842No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16260.1693575350042027No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15690.16342064724575278No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15680.16331649132016593No Hit
CCCCTAGGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA14150.14738063470537935No Hit
ACTTATTACTGCCTACACTATAATAGTTATTCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGA14110.146964011003032No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13990.14571413989598989No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC13970.1455058280448162No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC13710.14279777397955834No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13640.1420686825004505No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13570.14133959102134258No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13530.14092296731899523No Hit
CCTTATAGATCAGGAGCCTAGGGGCTTTCCCTGTTTTCTGCTGATACCAGGCCAACCAGCCACTAATACTCTGA13090.13634010659317425No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12720.13248633734646115No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12540.130611530685898No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12140.1264452936624244No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12130.12634113773683756No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCA12050.12550789033214282No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12040.12540373440655597No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11940.12436217515068759No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11400.1187377551689982No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11360.11832113146665084No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACT11340.11811281961547715No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGG11190.11655048073167455No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11010.11467567407111143No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10970.11425905036876406No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10890.11342580296406933No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10870.11321749111289564No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10760.11207177593144041No Hit
GCCTAGGGGCTTTCCCTGTTTTCTGCTGATACCAGGCCAACCAGCCACTA10650.11092606074998515No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10460.10894709816383519No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT10370.10800969483355362No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10270.10696813557768522No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG10260.10686397965209839No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10070.1048850170659484No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9990.10405176966125369No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9980.10394761373566684No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9850.1025935867030379No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9830.10238527485186423No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCCTAGGGGCTTTCCCTGTTTTCTG9660.10061462411688794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT208.6711196E-568.7447337
TATCGTA200.00743166151.62092238
CGAAAAT400.007714600351.13144770
ATGTCGT306.4272236E-445.8298237
TCGTATG2150.041.7002140
ATGCCGT2500.041.57445544
GCCGTCT2250.040.15799346
CCAATAC603.206933E-739.7544023
CTAGCGC3200.039.4961328
CGTATGC2550.039.27169841
GACTAGC3250.038.85604526
GTATCAA25250.038.7532271
CGAGACT3300.038.23739623
TAGCGCT3150.037.98795729
CCCACGA3500.037.9754519
CGCTCAT2850.037.27000832
ACTAGCG3300.037.24860827
ATCTCGT1850.037.15931737
ACGAGAC3450.036.5691822
CACGAGA3550.036.4919721
Produced by FastQC (version 0.11.5)