FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042648
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50230.4817541490512618No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49860.4782054921699365No Hit
GAGTATTAGTAATTGGTTGGCCTGGTATCAGCAGAAACTAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCTT42770.41020555355210964No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36490.3499742962150218No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35330.3388487773438399No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33830.3244623305276565No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33000.31650182995603504No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA32130.30815769080264865No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30970.2970321719314668No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGCTGATTTTGCAACTTA30070.2884003038417568No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA29850.28629029164204983No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28630.2745893148982207No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28620.2744934052527795No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28470.2730547605711611No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG27090.25981922950027236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26140.2507078131833562No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25740.2468714273657073No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25360.24322686083894082No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA24590.2358418181399667No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24320.2332522577130537No Hit
GGCCAAAAGTGTACACCGGAGAATAATTATTGTAGTGTTGGCAGTAATAAGTTGCAAAATCAGCAGGCTGCAGG23890.2291281429590811No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAATTGGTTGGCCTGGTATCAGCAGAAA23670.22701813075937421No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22980.22040036522392986No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22570.21646806976083974No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACAT21190.20323253868995095No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20940.2008347975539204No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20770.19920433358141962No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20220.19392930308215237No Hit
GTTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAGGCCAACCAATTACTA19590.18788699541935533No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19100.18318742279273542No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18840.18069377201126363No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18590.17829603087523307No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18480.17724102477537962No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18190.17445964505758416No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18180.17436373541214295No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18070.17330872931228947No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18040.17302100037596582No Hit
GCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAGGCCAACCAATTACTAATACTCTGA17570.16851324704022835No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACATCCAGATGACCCAGTCTCCTTCCACC17570.16851324704022835No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17430.16717051200405122No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA17410.16697869271316879No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16450.1577713667508114No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16450.1577713667508114No Hit
GTATTAGTAATTGGTTGGCCTGGTATCAGCAGAAACTAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCTTCTA16430.15757954745992894No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16300.15633272206919308No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16060.1540308905786037No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15600.14961904688830746No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15550.14913949866110135No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT15510.14875586007933647No Hit
CTATAAGGCTTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCT15400.14770085397948302No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15370.14741312504315934No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTAGAAGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTAGTTTCTG15180.1455908417797761No Hit
ACTTATTACTGCCAACACTACAATAATTATTCTCCGGTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15140.14520720319801123No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14950.14338491993462799No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC14790.14185036560756842No Hit
GTGTACACCGGAGAATAATTATTGTAGTGTTGGCAGTAATAAGTTGCAAA14410.13820579908080194No Hit
CTCCTGATCTATAAGGCTTCTACTTTAGAAAGTGGGGTCCCATCAAGGTT14320.13734261227183095No Hit
CCCCTAAACTCCTGATCTATAAGGCTTCTACTTTAGAAAGTGGGGTCCCA14310.13724670262638974No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT14270.13686306404462484No Hit
GATCAGGAGTTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAGGCCAACC14260.1367671543991836No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14030.13456123255403551No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATGTGGCACCTGGGAGCCAGAG13960.13388986503594694No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13700.13139621425447515No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13680.13120439496359268No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13660.13101257567271024No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13560.13005347921829805No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13430.12880665382756215No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13290.12746391879138502No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13270.12727209950050256No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13190.12650482233697277No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13040.12506617765535444No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGCTGATTTTG13030.12497026800991323No Hit
GTAATAAGTTGCAAAATCAGCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13000.12468253907358955No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12990.12458662942814833No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12920.12391526191005978No Hit
TTCTAAAGTAGAAGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTAGTT12640.12122979183770553No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATGTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG12590.12075024361049942No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12570.12055842431961698No Hit
GTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG12510.11998296644696964No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12500.11988705680152842No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12360.1185443217653513No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12350.11844841211991008No Hit
CTACAATAATTATTCTCCGGTGTACACTTTTGGCCAGGGGACCAAGCTGG12230.11729749637461541No Hit
CACCATCAGCAGCCTGCAGCCTGCTGATTTTGCAACTTATTACTGCCAACACTACAATAATTATTCTCCGGTGTA12220.11720158672917419No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11820.11336520091152529No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11520.11048791154828859No Hit
GGTATCAGCAGAAACTAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCTTCTACTTTAGAAAGTGGGGTCCCAT11400.10933699580299391No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11350.10885744757578782No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11330.10866562828490535No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11320.10856971863946413No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11310.10847380899402291No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11010.10559651963078621No Hit
GGCTTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCAT11010.10559651963078621No Hit
GTAGAAGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAGGCCAACCAATTACTAATA10820.103774236367403No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10720.10281513991299077No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10630.10195195310401976No Hit
GCCTGCAGCCTGCTGATTTTGCAACTTATTACTGCCAACACTACAATAAT10610.10176013381313732No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10580.10147240487681365No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG10480.10051330842240143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)