FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042648
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51100.4900982882046482No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39510.378939009138271No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39370.3775962741020939No Hit
GAGTATTAGTAATTGGTTGGCCTGGTATCAGCAGAAACTAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCTT38710.3712662375029732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36660.35160476018752257No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36260.3477683743698736No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30330.2908939546232286No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30310.29070213533234607No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA30080.288496213487198No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29750.2853311951876376No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29310.28111117078822384No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA28870.27689114638881007No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGCTGATTTTGCAACTTA28580.27410976667101455No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27420.26298424779983276No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25260.24226776438452863No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25220.24188412580276372No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24790.23776001104879116No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA24610.23603363743084915No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24320.2332522577130537No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG23390.22433266068701999No Hit
GGCCAAAAGTGTACACCGGAGAATAATTATTGTAGTGTTGGCAGTAATAAGTTGCAAAATCAGCAGGCTGCAGG23150.22203082919643063No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22570.21646806976083974No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAATTGGTTGGCCTGGTATCAGCAGAAA22410.21493351543378011No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22290.21378259968848548No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA22150.21243986465230832No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21950.2105216717434839No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21220.20352026762627465No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21210.2034243579808334No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20700.19853296606333107No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20630.1978615985452425No Hit
GTTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAGGCCAACCAATTACTA20380.1954638574092119No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACAT19970.19153156194612178No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19320.18529743499244233No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18650.1788714887478804No Hit
GCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAGGCCAACCAATTACTAATACTCTGA18400.17647374761184983No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18370.17618601867552616No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18270.17522692222111394No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17510.167937789167581No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACATCCAGATGACCCAGTCTCCTTCCACC17350.16640323484052144No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17180.16477277086802067No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17170.16467686122257943No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATGTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG16440.15767545710537018No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16330.15662045100551672No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16070.15412680022404493No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15590.14952313724286626No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15490.148564040788454No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15440.1480844925612479No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15440.1480844925612479No Hit
GTATTAGTAATTGGTTGGCCTGGTATCAGCAGAAACTAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCTTCTA15400.14770085397948302No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15140.14520720319801123No Hit
ACTTATTACTGCCAACACTACAATAATTATTCTCCGGTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15120.1450153839071288No Hit
GTGTACACCGGAGAATAATTATTGTAGTGTTGGCAGTAATAAGTTGCAAA15070.14453583567992265No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15060.14443992603448144No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14670.14069944986227376No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC14470.13878125695344928No Hit
CTATAAGGCTTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCT14280.13695897369006604No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14110.13532850971756527No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTAGAAGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTAGTTTCTG14080.1350407807812416No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14070.1349448711358004No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATGTGGCACCTGGGAGCCAGAG13970.13398577468138817No Hit
CTCCTGATCTATAAGGCTTCTACTTTAGAAAGTGGGGTCCCATCAAGGTT13950.1337939553905057No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13860.13293076858153471No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13780.13216349141800493No Hit
CCCCTAAACTCCTGATCTATAAGGCTTCTACTTTAGAAAGTGGGGTCCCA13670.13110848531815147No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13620.13062893709094536No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13520.12966984063653314No Hit
GTAATAAGTTGCAAAATCAGCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13500.1294780213456507No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12770.12247661722844143No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12740.12218888829211776No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12500.11988705680152842No Hit
CACCATCAGCAGCCTGCAGCCTGCTGATTTTGCAACTTATTACTGCCAACACTACAATAATTATTCTCCGGTGTA12360.1185443217653513No Hit
GATCAGGAGTTTAGGGGCTTTCCCTAGTTTCTGCTGATACCAGGCCAACC12340.11835250247446885No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12280.1177770446018215No Hit
GTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG12220.11720158672917419No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12220.11720158672917419No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12160.11662612885652685No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGCTGATTTTG11760.11278974303887794No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11750.11269383339343672No Hit
CTACAATAATTATTCTCCGGTGTACACTTTTGGCCAGGGGACCAAGCTGG11510.11039200190284737No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11500.11029609225740615No Hit
GGCTTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCAT11320.10856971863946413No Hit
TTCTAAAGTAGAAGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTAGTT11260.10799426076681679No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11260.10799426076681679No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11230.10770653183049314No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11230.10770653183049314No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11120.10665152573063967No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11120.10665152573063967No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG11100.10645970643975723No Hit
GGTATCAGCAGAAACTAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCTTCTACTTTAGAAAGTGGGGTCCCAT10980.10530879069446256No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10780.10339059778563811No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10530.10099285664960753No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG10490.10060921806784265No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10480.10051330842240143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)