FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785222
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT32080.4085468822829722No Hit
GTAATATACGGCCGTGTCCTCGCTTCTCAGGCTATTCATCTGCAAGTACA25150.32029158632845234No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23860.30386311132393135No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGTCCGAAATTGTAAGAGCCTGCATATGAG21050.2680770533683468No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17060.21726339812180506No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGTCCGAAATTGTAA14950.1903920165252629No Hit
GTATATTACTGTGTGAAAGGGGGGGGGTCTACCACCAATCCTTACGACCC14600.18593467834574173No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTGTGGGCTGAGCTGGCTTTTT14470.18427909559334812No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGACTGCTATCTGCCTTCCAG14320.18236880780212475No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG14210.18096793008856094No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13970.17791146962260354No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13820.17600118183138017No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13640.17370883648191213No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13240.16861473570531646No Hit
CCCCAGGGGGGCGCTTATTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA12970.16517621768111437No Hit
GATTATTACTGCAGCTCATATGCAGGCTCTTACAATTTCGGACTATTCGG12900.16428475004521015No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12620.1607188795015932No Hit
CAGTAATATACGGCCGTGTCCTCGCTTCTCAGGCTATTCATCTGCAAGTA12210.15549742620558263No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12010.1529503758172848No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11540.14696480740478488No Hit
CCCCTGGGGTCCCTGGTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG11390.1450545196135615No Hit
GTGCAGGGCCGGTTCATCATCTCCAGAGACAATTCCAGGAACACACTGTA11360.14467246205531684No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11340.14441775701648707No Hit
CTCCTGTGCAGCCTCTGGACTCACCTTTAGCGGATATGAGATGAGCTGGGTCCGCCAGGCTCCAGGGAGGGGG11240.14314423182233815No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11090.14123394403111478No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT10880.13855954112340205No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10850.13817748356515736No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGGAGTGACGTT10560.1344842605021255No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10340.1316825050749979No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10240.13040897988084899No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9980.1270978143760618No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9950.12671575681781713No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9900.12607899422074267No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC9830.12518752658483842No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9670.12314988627420016No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9520.12123959848297679No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9510.12111224596356189No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9510.12111224596356189No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9450.12034813084707255No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9370.11932931069175341No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9360.11920195817233853No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9310.11856519557526407No Hit
CTCCTGCACTGGAACCAGGAGTGACGTTGGTGGTTATACATATGTCTCCTGGTACCAACAACACCCAGGCAAAGC9160.11665490778404068No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGACTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8990.11448991495398753No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGTCCGAAATTGTAAG8980.11436256243457264No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8980.11436256243457264No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8920.11359844731808329No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8850.11270697968217905No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8750.11143345448803013No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8650.11015992929388123No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8600.10952316669680677No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGGAGTGACGTTGGTGGTTATACATATGTCTCCTGGTACCAACAACA8380.10672141126967916No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8140.10366495080372175No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8070.10277348316781751No Hit
GCCCTGCACAGAGTCTGCGTAGTAGGCCCTACTTCCGATACCGCTAATACTTGAGACCCACTCCAGCCCCCTCC8020.10213672057074305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCGA259.634383E-486.4204870
TTGAGCG200.00261068267.2291869
TTTAGTA200.00764652451.24918736
TACCGGG200.00809456550.5163127
CGTGCCG200.00811091950.49052426
TGGTATC1050.044.7890132
CTCTTAT2350.042.902221
GGTATCA4250.041.118831
TATACAC7500.040.7583
ATGGGGG1953.6379788E-1240.6250170
CTTATAC7700.040.1535071
ATCTCGT6450.039.8260737
TCGTATG7450.039.35361540
TCTCGTA6700.038.92704838
CTACATC6750.038.7463333
CGTCTTC7650.038.61878648
CCGTCTT7650.038.53656847
GCCGTCT7550.038.50272846
TATTAGG350.001536345938.3905832
CTTGAAA7900.038.30846857
Produced by FastQC (version 0.11.5)