FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004689570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785222
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70920.9031840676904112No Hit
GTAATATACGGCCGTGTCCTCGCTTCTCAGGCTATTCATCTGCAAGTACA22890.29150991694068684No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21790.27750113980504876No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGTCCGAAATTGTAAGAGCCTGCATATGAG19780.25190328340265555No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGACTGCTATCTGCCTTCCAG16190.20618372893270948No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14760.18797231865637998No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG14670.18682614598164596No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTGTGGGCTGAGCTGGCTTTTT13540.1724353112877632No Hit
CCCCAGGGGGGCGCTTATTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA12780.16275651981223144No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12560.1599547643851038No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGTCCGAAATTGTAA12380.15766241903563577No Hit
GTATATTACTGTGTGAAAGGGGGGGGGTCTACCACCAATCCTTACGACCC12260.1561341888026571No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12240.1558794837638273No Hit
GATTATTACTGCAGCTCATATGCAGGCTCTTACAATTTCGGACTATTCGG12210.15549742620558263No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12010.1529503758172848No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGGAGTGACGTT11890.15142214558430608No Hit
CAGTAATATACGGCCGTGTCCTCGCTTCTCAGGCTATTCATCTGCAAGTA11800.15027597290957206No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11790.1501486203901572No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC11650.14836568511834872No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11450.14581863473005086No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11250.14327158434175302No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10920.1390689512010616No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10660.13575778569627442No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10650.13563043317685955No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT10550.13435690798271063No Hit
CTCCTGTGCAGCCTCTGGACTCACCTTTAGCGGATATGAGATGAGCTGGGTCCGCCAGGCTCCAGGGAGGGGG10530.13410220294388084No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10520.13397485042446594No Hit
GTGCAGGGCCGGTTCATCATCTCCAGAGACAATTCCAGGAACACACTGTA10400.13244662019148726No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10360.1319372101138277No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10320.1314278000361681No Hit
CCCCTGGGGTCCCTGGTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG10150.12926280720611497No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10130.12900810216728517No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9810.12493282154600865No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9810.12493282154600865No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9690.12340459131302994No Hit
CTCCTGCACTGGAACCAGGAGTGACGTTGGTGGTTATACATATGTCTCCTGGTACCAACAACACCCAGGCAAAGC9440.12022077832765765No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGACTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9410.11983872076941299No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9290.11831049053643429No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9200.11716431786170024No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9100.11589079266755135No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9030.11499932503164711No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8980.11436256243457264No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8890.11321638975983862No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGGAGTGACGTTGGTGGTTATACATATGTCTCCTGGTACCAACAACA8640.11003257677446633No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8640.11003257677446633No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8410.10710346882792383No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8400.10697611630850892No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8210.10455641843962599No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCTCTTACAATTTCGGACTATTCGGCGGAGGGACCAAG8110.10328289324547707No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7890.10048113781834947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)